Porphyromonas gingivalis promotes NLRX1 translocation to activate mitophagy in esophageal carcinoma cells
10.19405/j.cnki.issn1000-1492.2022.12.011
- Author:
Lingyun Sun
1
,
2
;
Xiang Yuan
2
;
Linlin Shi
2
;
Xiusen Zhang
2
;
Jinyu Kong
2
;
Dingyu Zhang
2
,
3
;
Shegan Gao
2
Author Information
1. Dept of Clinical Laboratory of The First Affiliated Hospital of Henan University of Science and Technology， College of Clinical Medicine of Henan University of Science and Technology，Luoyang 471003
2. Henan Key Laboratory of Cancer Epigenetics，The First Affiliated Hospital of Henan University of Science and Technology，Luoyang 471003
3. College of Basic Medicine of Henan University of Science and Technology，Luoyang 471003
- Publication Type:Journal Article
- Keywords:
esophageal cancer;
Porphyromonas gingivalis;
ULK1;
NLRX1;
mitophagy
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1908-1914
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the relationship between Porphyromonas gingivalis(Pg) and mitophagy in esopha- geal cancer cells，and to explore new therapeutic targets for esophageal cancer．
Methods :① Western blot was used to detect the phosphorylation of unc-51-like authophagy activating kinase1 (ULK1) in mitochondria of the Pg infected cells and immunohistochemical method was used to detect the correlation between the expression of Pg and the phosphorylation status of ULK1 in esophageal cancer tissues. ② Western blot，ICC and ELISA were used to de- tect the transfer of nucleotide blinding domain and leucine rich repeat containing family member X1 (NLRX1) from cytoplasm to mitochondria，mitophagy，and the secretion levels of interleukin ( IL) -6 and reactive oxygen species (ROS) under Pg infection. ③ Pg colonization in esophageal tissues of mice in each group was detected by qPCR and Pg colonization in esophageal squamous epithelial cells of mice by RNAscope.
Results :Compared with the un- treated group，the phosphorylation level of mitochondrial ULK1 (P＜0．01) ，NLRX1 expression (P＜0. 001) and mitophagy (P＜0. 001) of esophageal cancer cells increased after Pg infection．Compared with the control group， the combined intervention group could inhibit Pg colonization in esophageal tissue and esophageal squamous epithe- lial cells of mice (P＜0. 001) ．
Conclusion :Pg promotes the translocation of NLRX1 from cytoplasm to mitochon- dria by up-regulating the phosphorylation level of ULK1 in the mitochondria of esophageal cancer cells，and then induces mitophagy，leading to the reduction secretion of IL-6 and ROS，and ultimately maintaining Pg colonization.
- Full text:2024072116101743967牙龈卟啉单胞菌促进食管癌细...1转位激活线粒体自噬的研究_孙玲云.pdf
