Study on antidepressant mechanism of helicid based on network pharmacology
10.19405/j.cnki.issn1000-1492.2022.12.009
- Author:
Zhenyi Jiang
1
;
Yuan Zhang
1
;
Zepeng Li
1
;
Xiaotong Zhang
1
;
Yuanxiang Zhang
1
;
Jiucui Tong
2
Author Information
1. The Graduate School of Wannan Medical College，Wuhu 241002
2. Clinical Pharmacy Department of Yijishan Hospital of Wannan Medical College，Wuhu 241001
- Publication Type:Journal Article
- Keywords:
helicid;
depression;
network pharmacology;
drug targets
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1896-1901
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To screen the target of helicid in the intervention of depression based on network pharmacol- ogy and molecular docking，and to study the effect of helicid on the expression level of related targets in hippocam- pus，prefrontal cortex，striatum and habenular nucleus of chronic unpredictable mild stress ( CUMS) rats．
Methods :The target of helicid was predicted by SwissTargetPrediction database ，and the depression related targets were screened by GeneCards、DisGeNet、TTD and DRUGBANK databases ; the metascape platform was used for gene en- richment analysis ，and the " helicid-depression-pathway " network was constructed ; Autodock Vina was used for molecular docking research ; qRT-PCR was used to detect the effect of helicid on the mRNA expression of HTR1A， ADORA1 and ADORA2A in rat tissues.
Results :The 81 helicid targets and 1 640 depression targets were ob- tained，including 40 intersecting targets ; the key targets were mainly enriched in cAMP signal pathway，PI3K-Akt signal pathway，MAPK signal pathway and so on ; the results of molecular docking showed that the binding activity of helicid with most targets was good ; helicid up-regulated the expression levels of HTR1A ，ADORA1 and ADORA2A mRNA in hippocampus，prefrontal cortex ，striatum and habenular nucleus of CUMS rats．
Conclusion:Helicid may act on cAMP，PI3K-Akt，MAPK and other signal pathways to intervene depression through HTR1A， ADORA1 and ADORA2A.
- Full text:2024072115534249348基于网络药理学研究豆腐果苷抗抑郁作用机制_蒋臻熠.pdf
