Expression of programmed death 1 on CXCR5 － CD4 + T cell from the patients with rheumatoid arthritis and clinical significance
10.19405/j.cnki.issn1000-1492.2022.12.008
- Author:
Qing Luo
1
;
Lina Tu
2
;
Biqi Fu
3
;
Xue Li
1
;
Junming Li
1
Author Information
1. Dept of Clinical Laboratory，The First Affiliated Hospital of Nanchang University，Nanchang 330006
2. Dept of Medical Technology，Nanchang Medical College，Nanchang 330052
3. Dept of Rheumatology，The First Affiliated Hospital of Nanchang University，Nanchang 330006
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
CXCR5 －CD4 + T cells;
PD1
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1891-1895
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of programmed death 1 (PD1) on CXCR5 －CD4 + T cells from the patients with rheumatoid arthritis (RA) and to analyze the clinical relevance to disease severity．
Methods :The expression of PD1 on CXCR5 －CD4 + T cells were examined from 82 RA patients and 46 healthy controls (HC) by the technique of flow cytometry．The expression of PD1 including mean fluorescence intensity (MFI) on CXCR5 - CD4 + T cells and percentage of PD1 + CXCR5 －CD4 + T cells were compared between RA patients and HC．Moreo- ver，its correlation with laboratory inspection was analyzed.
Results :① The percentage of PD1 + CXCR5 －CD4 + T cells and the MFI of PD1 on CXCR5 －CD4 + T cells from RA patients were significantly elevated compared with HC (P＜0. 000 1，U = 1 082 ; P ＜0. 000 1 ，U = 917. 5) . ② The percentage of PD1 + CXCR5 － CD4 + T cells in RA patients were positively associated with rheumatoid factors (RF) (rs = 0. 267 9，P = 0. 022 9) ，increased RF (rs = 0. 419，P = 0. 001 1) ，increased anti-cyclic citrullinated peptide (anti-CCP) (rs = 0. 260 7，P = 0. 040 7) ，the percentage of plasmablasts (rs = 0. 302 4，P = 0. 029 3) ，DAS28-ESR (rs = 0. 244 7，P = 0. 042 7) . ③ The MFI of PD1 on CXCR5 －CD4 + T cells in RA patients were negatively with lymphocytes number (rs = －0. 338 7，P = 0. 002 0 ) ，lymphocytes percentage ( rs = －0. 347 9 ，P = 0. 001 4 ) ，lymphocyte-to-monocyte ratio ( rs = - 0. 263 7，P = 0. 017 4) ．The MFI of PD1 on CXCR5 － CD4 + T cells in RA patients were positively associated with neutrophil percentage ( rs = 0. 304 7 ，P = 0. 005 4 ) ，neutrophilto-lymphocyte ratio ( rs = 0. 341 1 ，P = 0. 001 8) ，platelet-to-lymphocyte ratio (rs = 0. 232 1，P = 0. 037 1) ，systemic immune inflammation index (rs = 0. 288 0，P = 0. 009 1) ，derived Neutrophil to lymphocyte ratio ( rs = 0. 320 3，P = 0. 003 6) ，erythrocyte sedi- mentation rate (rs = 0. 259 5，P = 0. 019 3) ，patient visual analogue scale (rs = 0. 241 6，P = 0. 043 9) ．The MFI of PD1 on CXCR5 － CD4 + T cells from high active RA patients was significantly elevated compared with non-high active group (P = 0. 040 7，U = 406. 0) ．
Conclusion :The abnormal expression of PD1 on CXCR5 －CD4 + T cells are observed in patients with RA．Increased expression of PD1 on CXCR5 － CD4 + T cells are associated with the production of antibody，the percentage of plasmablasts，inflammation，and also disease activity.
- Full text:2024072115495370940类风湿关节炎患者外周血CX...+T细胞上PD1表达及意义_罗清.pdf
