The analysis of TMOD1 interacting proteins associated with macrophage migration
10.19405/j.cnki.issn1000-1492.2022.12.007
- Author:
Siyu Jiang
1
,
2
;
Shan Ma
1
,
2
;
Bo Yuan
1
;
Lide Xie
1
;
Weijuan Yao
2
;
Lina Guo
3
,
4
Author Information
1. Dept of Basic Medical School，Chengde Medical University，Chengde 067000
2. Dept of Basic Medical School，Physiology and Pathophysiology，Hemorheology Center， Peking University，Beijing 100083
3. Dept of Rehabilitation，Cao County People '
4. s Hospital，Heze 274400
- Publication Type:Journal Article
- Keywords:
macrophages;
TMOD1;
interaction;
mass spectrometry;
GeneCards database
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1885-1890
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To analyze interacting proteins of tropomodulin1 (TMOD1 ) in Raw264．7 mouse monocyte macrophage line by mass spectrometry and GeneCards database．
Methods :Immunoprecipitation combined with mass spectrometry was used to find interacting proteins of TMOD1 after overexpress TMOD1 in Raw264．7 cells. GeneCards database was used to search for known genes for macrophage migration．Bioinformatics ＆ Systems Biolo- gy was used to analyze correlation between known targets and mass spectrometry proteins to find common differenti- ally expressed proteins( CO-DEPs) ．WoLF PSORT was used to predict subcellular localization of CO-DEPs．Egg- NOG databasewas used to analyze eukaryotic orthologous group(KOG) of CO-DEPs．DAVID database was used to analyze gene ontology( GO) enrichment kyoto encyclopedia of genes and genomes( KEGG) pathway of CO-DEPs. String database was used to analyze protein interaction network and CytoScape software drawing.
Results :There were 41 CO-DEPs in mass spectrometry and GeneCards database．Subcellular localization of CO-DEPs was mainly distributed in cytoplasm，nucleus and mitochondria．KOG notes were mainly O : post-translational modification，Z : cytoskeleton and J : translation．GO enrichment found that CO-DEPs was mainly involved in poly (A) RNA bind- ing，protein folding and focal adhesion．KEGG was mainly enriched in arrhythmogenic right ventricular cardiomyop- athy (ARVC) and tight junction．ACTB was a protein with large protein interaction．
Conclusion :The proteins in- teracting with TMOD1 in macrophages mainly include myosin heavy chain-9 (MYH9) ，α-actinin 1 (ACTN1) and β-actin (ACTB) ，etc，suggesting that TMOD1 is related to macrophages migrate.
- Full text:2024072115453893677巨噬细胞中与原肌球蛋白调节...互作用的迁移相关蛋白质分析_江思瑜 (1).pdf
