The role of endoplasmic reticulum stress in hepatic insulin resistance caused by intermittent hypoxia
10.19405/j.cnki.issn1000-1492.2022.12.006
- Author:
Huanhuan Jin
1
;
Tingting Sun
1
;
Lijue Ren
1
;
Zhongming He
2
;
Xinjie Chen
1
;
Jiayu Hu
1
;
Fang Han
3
,
4
;
Cuiying Wei
1
Author Information
1. Dept of Geriatrics，The First Affiliated Hospital of Baotou Medical College， Inner Mongolia University of Science and Technology，Baotou 014000
2. Dept of Respiratory Medicine，Karamay Central Hospital，Xinjiang，Karamay 834000
3. Sleep Center，Dept of Respiratory Medicine，Peking University People '
4. s Hospital，Beijing 100000
- Publication Type:Journal Article
- Keywords:
intermittent hypoxia-reoxygenation;
insulin resistance;
endoplasmic reticulum stress;
unfolded protein response
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1880-1885
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the role of endoplasmic reticulum stress in hepatic insulin resistance induced by intermittent hypoxia in rats．
Methods :Twenty-four SD rats were randomly divided into control group ( NC group) and intermittent hypoxia group ( CIH group) ．The NC group was placed in a normoxia environment for 12 weeks，and the CIH group was given intermittent hypoxia for 8 weeks，and then returned to normoxia until the 12th week．In both groups，fasting blood glucose (FBG) ，fasting insulin (FINS) ，and liver inositol-requiring enzyme- 1 α(IRE1 α) ，X-box binding protein 1s(XBP1s) ，forkhead box transcription factor O1 (FoxO1) ，activating transcription factor-6(ATF6) ，cAMP-response element binding protein( CREB) ，CREB-regulated transcription coacti- vator-2( CRTC2) ，double-stranded RNA-dependent protein kinase-like ER kinase ( PERK) ，eukaryotic initiation factor 2 α(eIF2 α) ，protein kinase B ( AKT) ，phosphoenolpyruvate carboxykinase ( PEPCK) ，glucose-6-phosphat- ase( G6Pase) mRNA were measured at baseline，week 8，and week 12 .
Results :There was no significant differ- ence in each observation index between the two groups at baseline ; at 8 weeks，the levels of FBG，FINS and the mRNA levels of IRE1α , XBP1s，ATF6，PERK，eIF2 α , PEPCK and G6Pase in the CIH group were higher than those in the NC group (P＜0. 05) ，while the mRNA levels of CREB，CRTC2 and AKT were lower than those in the NC group (P＜0. 05) ; at 12 weeks，there was no significant difference in each observation index between the two groups．Pearson correlation analysis showed(8th week of intermittent hypoxia group) : homeostasis model as- sessment-insulin resistance(HOMA-IR) was positively correlated with FoxO1，CREB，CRTC2 and PERK，eIF2 α mRNA levels (r = 0. 172，0. 595，0. 183，0. 702，0. 608 ; P＜0. 05) while it was negatively correlated with IRE1α , XBP1s，ATF6，AKT mRNA levels (r = －0. 422 ，－0. 327 ，－0. 309 ，－0. 399 ; P＜0. 05) ．
Conclusion :Intermittent hypoxia can lead to insulin resistance，and endoplasmic reticulum stress may mediate this effect.
- Full text:2024072115352489435内质网应激在间歇低氧所致肝脏胰岛素抵抗中的作用_靳欢欢.pdf
