Expression of LINC01152 in glioma and its effect on malignant biological behavior of glioma cells
10.19405/j.cnki.issn1000-1492.2023.04.017
- Author:
Xin Li
1
;
Chang Liu
1
,
2
;
Chunhong Xue
1
;
Ping Wang
1
;
Feng Li
1
;
Yingying Ge
1
,
3
;
Weixia Nong
1
,
3
;
Qingmei Zhang
1
,
3
;
Xiaoxun Xie
1
,
3
;
Bin Luo
1
,
3
Author Information
1. Dept of Histology and Embryology，School of Basic Medicine Sciences，Guangxi Medical University，Nanning 530021
2. The First Affiliated Hospital of Guangxi Medical University，Nanning 530021
3. Key Laboratory of Basic Medical Research of Guangxi Colleges and Universities，Nanning 530021
- Publication Type:Journal Article
- Keywords:
IncRNA;
glioma;
malignant biological behavior
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):622-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of long non-coding RNA LINC01152 in glioma and its influence on the malignant biological behavior of glioma cells．
Methods:LINC01152 expression in glioma was analyzed by LncSpA V2．0 and GEPIA database．qRT-PCR was applied to detect the expression of LINC01152 mRNA in 10 samples of human normal brain tissues，40 samples of glioma tissues and 5 glioma cell lines．GO and KEGG enrichment analysis of LINC01152 co-expressed genes were performed using the DAVID database to predict the related functions． The AnoLnc2，TargetScan，LinkedOmics and miRDB databases were used to predict the LINC01152 related miRNAs and target genes to construct a ceRNA regulatory network．LINC01152 expression was knocked down in glioma cell lines by small interfering RNA (siRNA) transfection．The CCK-8 test，scratch healing experiments，Transwell，flow cytometry and Western blot experiments were used to measure the influence of LINC01152 on the proliferation，migra- tion，invasion and apoptosis of glioma cells.
Results :Database analysis showed that compared with other tumor types，LINC01152 was highly expressed in glioblastoma (GBM) and low grade glioma (LGG) ，and was higher than normal brain tissue．qRT-PCR showed that the expression of LINC01152 mRNA in glioma tissues was significantly higher than that in normal brain tissues (P＜0. 01)．The expression of LINC01152 was correlated with Ki-67 (P < 0. 05) ，but not with clinical parameters such as gender，age，tumor size，P53 protein，glial fibrillary acidic protein (GFAP) ，O-6-methylguanine-DNAmethyltransferase ( MGMT) and WHO grade of glioma patients． Functional enrichment analysis of co-expressed genes indicated that the LINC01152 was mainly involved in biological processes such as cell adhesion and synaptic signaling．LINC01152-miRNA-mRNA regulatory network was constructed according to predicted target genes．After down-regulation of LINC01152 expression，the proliferation，migration and invasion abilities of A172 and U87 cells decreased(P＜0. 01) ，while the apoptosis of glioma cells significantly increased (P＜0. 001) ．
Conclusion:LINC01152 is highly expressed in glioma，which can promote the malignant biological behavior of glioma cells by enhancing proliferation，migration as well as invasion and inhibition of apoptosis.
- Full text:2024071722222267959胶质瘤中LINC01152...瘤细胞恶性生物学行为的影响_李鑫.pdf
