Effects of hsa-miR-642b-3p on cerebral microvascular endothelial cells under hypoxia/reoxygenation
10.19845/j.cnki.zfysjjbzz.2022.0268
- VernacularTitle:hsa-miR-642b-3p对缺氧/复氧条件下脑微血管内皮细胞的作用研究
- Author:
Yan ZHAO
1
;
Xiaobo YANG
1
Author Information
1. Department of Clinical Laboratory,Jinshan Hospital,Fudan University,Shanghai 201508,China
- Publication Type:Journal Article
- Keywords:
Ischemic stroke;
Hypoxia/reoxygenation;
Endothelial cell;
microRNA;
Bioinformatic analysis
- From:
Journal of Apoplexy and Nervous Diseases
2022;39(12):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of hsa-miR-642b-3p on cerebral microvascular endothelial cells under hypoxia/reoxygenation (H/R).Methods The H/R model of brain microvascular endothelial cells was established to analyze the expression alteration of hsa-miR-642b-3p,and the intervention was carried out accordingly.Cell death was detected by trypan blue staining,cell proliferation was detected by CCK8,cell permeability was detected by FITC-albumin,and potential GO and KEGG function enrichment of hsa-miR-642b-3p was analyzed by bioinformatics.Results The expression of hsa-miR-642b-3p was elevated in H/R condition.After administration of hsa-miR-642b-3p inhibitor,we found the cell death was reduced,cell proliferation was increased and cell permeability was decreased under H/R condition.In addition,GO and KEGG functional enrichment analysis showed that hsa-miR-642b-3p may be involved in multiple biological functions and signal pathways such as RNA regulation,cell adhesion,TGF-β pathway and others.Conclusion Abnormal expression of hsa-miR-642b-3p in cerebral microvascular endothelial cells under H/R may have certain value for ischemic stroke.
- Full text:2024071721572442417Effects of hsa-miR-642b-3p on cerebral microvascular endothelial cells under hypoxia_reoxygenation.pdf
