Effect of RORγ gene on proliferation and migration of colon cancer cells
- Author:
Changyi Fang
1
;
Xiaofei Pan
2
,
3
;
Changjun Yu
1
;
Ping Wu
2
,
3
;
Benxin Chen
2
,
3
;
Bao Li
2
,
3
;
Gan Zhang
2
,
3
;
Yuyong Gong
2
,
3
;
Yang Li
2
,
3
Author Information
- Publication Type:Journal Article
- Keywords: colon cancer; retinoid-related orphan receptor γ; immune infiltration; proliferation; migration
- From: Acta Universitatis Medicinalis Anhui 2023;58(4):603-608
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of retinoid-related orphan receptor γ (RORγ) gene on proliferation and metastasis of human colon cancer cells．
Methods:RORγ knockdown cell lines were constructed and the knockdown efficiency was detected by RT-qPCR and Western blot assays ; MTT，colony formation，Transwell and wound healing assays were used to detect cell proliferation and metastasis ; the expression of epithelial-mesenchymal transition (EMT) related proteins was detected by Western blot．The relationship between RORγ gene expression and immune cell infiltration in tumor microenvironment was analyzed using TIMER 2. 0 database.
Results :The knockdown of RORγ enhanced the viability (F = 157. 40，P＜0. 01) ，clonogenesis (F = 61. 35，P＜0. 01) ，migration (F = 13. 00，P＜0. 01) ，invasion (F = 21. 26，P＜0. 01) and wound healing ability (F = 877. 2，P＜0. 01) of colon cancer cells，inhibited the expression of E-Cadherin，and promoted the expression of vimentin and N-Cadherin．TIMER 2. 0 database analysis showed that RORγ expression in colon adenocarcinoma ( COAD) tissues was associated with multiple immune cell infiltrates．
Conclusion:Downregulation of RORγ expression promoted the proliferation and metastasis of colon cancer cells. - Full text:2024071721314612323RORγ基因对结肠癌细胞增殖及迁移的影响_方昌义.pdf
