The roles of Bajitianwan formula on Aβ-injured osteoblasts and the mechanism based on network pharmacology
10.12206/j.issn.2097-2024.202305011
- VernacularTitle:巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究
- Author:
Tao JIANG
1
,
2
;
Weifan XU
1
,
2
;
Yiping JIANG
2
;
Tianshuang XIA
2
;
Hailiang XIN
1
,
2
Author Information
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2. Department of Pharmacognosy, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
osteoblasts;
Bajitianwan;
Aβ deposition;
network pharmacology
- From:
Journal of Pharmaceutical Practice and Service
2024;42(7):285-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Bajitianwan（BJTW）formula on bone formation of Aβ-injured osteoblasts and its mechanism. Methods Osteoblasts isolated from neonatal 24-hour Wistar rats were used for the study, and osteoblasts were subjected to damage with Aβ1-42 oligomers, and pharmacological intervention was performed with the aqueous extract of BJTW formula. The MTT assay, alkaline phosphatase（ALP）activity assay, catalase（CAT）activity assay, superoxide dismutase（SOD）activity assay, glutathione（GSH）activity assay and malondialdehyde（MDA）activity assay were carried out respectively. The expression levels of bone morphogenetic protein 2（BMP2）, osteogenic specific transcription factor（RUNX-2）and osteoprotective protein（OPG）were detected by Western blotting. After confirming the effect of BJTW formula on Aβ-injured osteoblasts, the network pharmacology method was used to predict the potential pathways. Results The BJTW formula significantly promoted the proliferation of Aβ-injured osteoblasts, increased ALP, SOD and GSH activity, inhibited MDA activity, and promoted the expression of bone formation-related proteins BMP2, RUNX-2 and OPG. Network pharmacological analysis showed that the effect of ameliorating of Aβ-injured osteoblasts by BJTW formula was mainly mediated by AGE-RAGE, PI3K-Akt, MAPK and neuroactive ligand-receptor interaction signaling pathways. Conclusion In this study, the effect of BJTW formula on improving the osteoblasts damaged by Aβ was confirmed for the first time, and its related mechanism was explored based on network pharmacology method. The results lay a strong foundation for the clinical application of traditional formula BJTW against osteoporosis.
