Current status and progress of single cell RNA sequencing in the cellular mechanisms of diabetic retinopathy
10.3980/j.issn.1672-5123.2024.8.16
- VernacularTitle:单细胞转录组测序技术在糖尿病视网膜病变中细胞机制的研究现状和进展
- Author:
Jianing YING
1
,
2
;
Yanyan ZHANG
1
,
2
;
Quanyong YI
1
,
2
Author Information
1. Health Science Center, Ningbo University, Ningbo 315211, Zhejiang Province, China
2. Ningbo Eye Hospital, Ningbo 315040, Zhejiang Province, China
- Publication Type:Journal Article
- Keywords:
single-cell RNA sequencing;
diabetic retinopathy;
cellular mechanism;
heterogeneity
- From:
International Eye Science
2024;24(8):1266-1269
- CountryChina
- Language:Chinese
-
Abstract:
Diabetic retinopathy(DR)is one of the most common retinal complications of diabetes could cause irreversible loss of central vision in the working-age population. Current studies showed that systemic risk factors, inflammatory response, and oxidative stress played a central role in the development of DR. Although traditional sequencing methods have provided valuable insights into the pathogenesis of DR, offering crucial guidance for clinical diagnosis and treatment, they still possess certain limitations. In recent years, the emerging single-cell RNA sequencing technology(scRNA-seq)has enabled precise analysis of mRNA transcriptomes at the single-cell level. This technique accurately identifies novel cell subtypes in retinal diseases, detects rare cells, and reveals intercellular heterogeneity. It contributes to elucidating the pathogenesis and development of retinal diseases, and facilitates exploration of gene regulatory relationships associated with these disorders to provide valuable insights for future precision medicine. This article reviews the technology of single-cell sequencing and its application in DR research. It also explores the mechanisms of different types of cells associated with DR, aiming to enhance the utilization of scRNA-seq in DR research and identify potential therapeutic targets to improve clinical diagnosis and treatment of DR.
