Effect of CTRP9 on lipid metabolism in brown adipose tissue of mice induced by cold stimulation
10.19405/j.cnki.issn1000-1492.2023.04.010
- Author:
Hua Guan
1
,
2
,
3
,
4
,
5
,
6
;
Huanhuan Chang
7
,
8
,
9
,
10
,
11
;
Xiangyu Li
5
,
6
,
12
;
Xue Wang
10
,
13
,
14
;
Yang Gao
10
,
13
,
14
;
Junjun Hao
10
,
13
,
14
;
Fengwei Guo
10
,
13
,
14
;
Tao Shi
10
,
13
,
14
Author Information
1. Laboratory for Lipid Metabolism and Atherosclerosis，Xi '
2. an Jiaotong University Cardiovascular Research Center， Xi '
3. an 710061
4. Institute of Basic and Translational Medicine of Xi '
5. an Medical University，Xi '
6. an 710021
7. Dept of Otolaryngology，Xi '
8. an Children '
9. s Hospital Affiliated to Xi '
10. an Jiaotong University，Xi '
11. an 710003
12. Institute of Basic and Translational Medicine of Xi '
13. Dept of Cardiovascular Surgery，The First Affiliated Hospital of Xi '
14. an 710061
- Publication Type:Journal Article
- Keywords:
cold stimulation;
CTRP9;
brown adipose tissue;
UCP-1;
ATGL
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):577-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of C1q / tumor necrosis factor-related protein 9 ( CTRP9 ) on the expression of genes and proteins related to lipid metabolism of brown adipose tissue (BAT) in mice after cold stimulation．
Methods :C57BL /6J male mice were injected with adenovirus Ad-GFP (control group) or Ad-CTRP9 ( experience group) into the scapular region and kept for 7 days．After cold stimulation at 4 ℃ for 10 hours，the expression levels of BAT marker genes and proteins were detected by real time PCR and Western blot.
Results:Overexpression of CTRP9 induced by cold stimulation significantly increased the mRNA level of iodothyronine deiodinase 2 (Dio2) in BAT (P＜0. 01) ．Additionally，there was no significant difference in the expression of BAT marker genes ( UCP-1，PGC-1 α , PRDM16 and ARβ3) ，and liposynthesis and lipolysis related genes (PPARγ , HSL and ATGL) ．Uncoupling protein 1 (UCP-1) protein expression was upregualted in Ad-CTRP9 compared to the Ad-GFP control group ，while the expression of lipolysis related protein adipose triglyceride lipase ( ATGL) decreased significantly (P＜0. 05) ．
Conclusion:In cold environment，overexpression of CTRP9 promotes the accumulation of UCP-1 protein in BAT，upregulates the expression of thyroid hormone signal related gene Dio2，and inhibits triglyceride hydrolysis to maintain a constant body temperature.
- Full text:2024071710373162036CTRP9对冷刺激诱导的小...棕色脂肪组织脂质代谢的影响_关华.pdf
