Relevance between hypocitraturia and VDR gene promoter methylation of the Bai nationality in Yunnan province
10.19405/j.cnki.issn1000-1492.2023.04.009
- Author:
Xiaowei Lin
1
;
Yuhui Luo
1
;
Jingling Li
1
;
Baiyu Zhang
1
;
Kunbin Ke
1
;
Hao Li
1
Author Information
1. Dept of Urology，the First Affiliated Hospital of Kunming Medical University，Kunming 650032
- Publication Type:Journal Article
- Keywords:
vitamin D receptor;
gene promoter;
DNA methylation;
idiopathic hypocitraturia
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):573-576
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the relevance between vitamin D receptor ( VDR) gene promoter methylation level and idiopathic hypocitraturia of the Bai nationality in Yunnan province．
Methods :Fifteen patients with idiopathic hypocitrouria of the Bai nationality in Yunnan province with double dominant expression (FF type) of single nucleotide polymorphism shot (SNP shot) rs2228570 (Fok Ⅰ ) genotype were selected as the experimental group. Fifteen people of the Bai nationality in Yunnan province with normal content of urinary citric acid were the control group．First，blood samples were taken from both groups．Next，the blood samples were treated with sulfites，RNA products of each sample were obtained by PCR amplification and in vitro transcription of T7 DNA polymerase．Then the corresponding RNA fragments were digested by base-specific enzymes．Finally the degree of methylation at each test site was obtained through the EpiTYPER procedure.
Results:In the statistical results of DNA methylation level，the methylation level of VDR Ⅰ fragment experimental group was 4. 136% ( 1. 655% ，5. 152% ) which was higher than 1. 261% (0. 827% ，1. 930% ) in the control group，and the difference was statistically significant (P ＜0. 001) ．Among the 33 CpG sites on VDR Ⅰ fragment，there were significant differences in DNA methylation levels of CPG-5 (F = 8. 831，P = 0. 008) and CpG-8 (F = 16. 155，P = 0. 001) between the experimental group and the control group．
Conclusion:The increased methylation level of VDR gene promoter is related with idiopathic hypocitric of the Bai nationality of Yunnan province．And compared with the normal Bai nationality people，the DNA methylation level of VDR gene promoter significantly increased in the Bai nationality patients with FF type of VDR gene SNP shot Fok Ⅰ .
- Full text:202407171031205903云南白族低枸橼酸尿症与VD...基因启动子甲基化水平的关系_林晓伟.pdf
