Effects of ASIC1 knockout on articular cartilage injury in adjuvant arthritis of mice
10.19405/j.cnki.issn1000-1492.2023.04.008
- Author:
Beibei Dai
1
;
Shengqin Zu
2
;
Renpeng Zhou
2
;
Feihu Chen
2
Author Information
1. Dept of Pharmacy，The Third Grade Laboratory of Traditional Chinese Medicine Chemistry of State Administration of Traditional Chinese Medicine，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. School of Pharmacy，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
ASIC1;
adjuvant arthritis;
articular cartilage;
rheumatoid arthritis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):567-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of acid-sensing ion channel 1 (ASIC1) gene knockout on articular cartilage injury in adjuvant arthritis (AA) of mice．
Methods:Wild-type mice and ASIC1 knockout mice were divided into normal group and model group．Arthritis score was performed by observing the inflammation of paws，and the right paw swelling was measured．HE staining，immunohistochemistry，TUNEL and ELISA were used to detect the injury of articular cartilage and arthritic inflammation.
Results :The arthritis score and paw swelling of AA mice with ASIC1 knockout was lower than that of the AA wild-type mice．AA mice with ASIC1 knockout showed less destruction and increased expression of collagen-Ⅱin articular cartilage． Moreover ，the lower apoptosis rate was observed by TUNEL assay in AA mice with ASIC1 knockout by comparing with AA wild-type mice．Furthermore，ELISA assay showed that the levels of IL-1 β、TNF-α in the serum decreased in AA mice with ASIC1 knock- out．
Conclusion:Knockout of ASIC1 may have protective effect on articular cartilage injury.
- Full text:2024071710263361632ASIC1基因敲除对佐剂性...节炎小鼠关节软骨损伤的影响_代贝贝.pdf
