Effects of exosome －derived LncRNA ESCCAL－1 /miR - 874 / ITGBL1 on the proliferation and apoptosis of colorectal cancer cells
10.19405/j.cnki.issn1000-1492.2023.03.016
- Author:
Ermin Ma
1
;
Zhaohong Zhang
1
;
Jingjing Huang
1
;
Xiang Liu
1
;
Geng Chi
1
;
Lei Liu
2
;
Nan Zhang
1
Author Information
1. General Surgery，The First Affiliated Hospital of Henan University of Traditional Chinese Medicine，Zhengzhou 450099
2. Dept of Pathology，The First Affiliated Hospital of Henan University of Traditional Chinese Medicine，Zhengzhou 450099
- Publication Type:Journal Article
- Keywords:
LncRNA ESCCAL-1;
miR-874;
ITGBL1;
colorectal cancer
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):442-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of exosome-derived LncRNA ESCCAL-1 regulating the miR-874 /ITGBL1 axis in the progression of colorectal cancer ( CRC) ．
Methods :The differentially expressed genes in CRC were analyzed using the Gene Expression Omnibus( GEO) database．Expressions of LncRNA ESCCAL-1，miR-874 and ITGBL1 in CRC tissues and cell lines (SW480，SW620，HCT116 and HT29) and adjacent normal tissues and NCM460 cell lines were detected by qRT-PCR ; 3-( 4，5-dimethylthiazol-2-yl ) -2，5diphenyltetrazoliumbromide (MTT) ，clone formation and flow cytometry was used to detect cell proliferation，colony formation and apoptosis ; dual luciferase reporter assays were used to verify the interaction between miR-874 and ESCCAL-1，ITGBL1 ; fluorescence in situ hybridization was used to determine the subcellular localization of LncRNAESCCAL-1 ．Exosomes were isolated from serum using the Exosome extraction kit.
Results :The expressions of ESCCAL-1 and ITGBL1 in CRC tissues and cell lines were higher than those in adjacent normal tissues and NCM460 cell lines，while the opposite was true for miR-874 (P＜0. 05) ．Knockdown of ESCCAL-1 can inhibit CRC cell proliferation and colony formation and promote apoptosis．There are specific binding sites formiR-874 and ESCCAL-1，and miR-874 inhibitor could partially reverse the effect of knockdown ESCCAL-1 in CRC (P＜0. 05) ．ESCCAL-1 upregulates ITGBL1 by adsorbing miR-874．The serum levels of ESCCAL-1 and exo-ESCCAL-1 in CRC patients were higher than those in the control group．Serum exo-ESCCAL-1 may be a valuable diagnostic indicator for CRC treatment (P＜0. 05) .
Conclusion :ESCCAL-1 promotes CRC progression by regulating the miR-874/ ITGBL1 axis，and ESCCAL-1 may be an effective molecular target for CRC therapy.
- Full text:2024071620470178552外泌体源性LncRNA_E...结直肠癌细胞增殖凋亡的影响_马二民.pdf
