Lycorine alleviates oxaliplatin－induced chemotherapy induced pain in mice via reducing COX－2 /NLRP3 inflammatory signaling
10.19405/j.cnki.issn1000-1492.2023.03.014
- Author:
Chenlu Guo
1
;
Ji Wu
2
;
Heyu Yang
1
;
Min Xie
1
;
Ling Liu
1
Author Information
1. School of Pharmacy，Xianning Medical College，Hubei University of Science and Technology，Xianning 437100
2. Affiliated Hospital of Youjiang Medical University for Nationalities，Baise 533000
- Publication Type:Journal Article
- Keywords:
lycorine;
chemotherapy induced pain;
neuroinflammation;
cyclooxygenase-2
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):429-433，441
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of lycorine on oxaliplatin ( OXA) induced chemotherapy pain in mice．
Methods :40 mice were randomly divided into 4 groups，10 mice per group，which were respectively divided into control group，model group，administration group，and inhibitor group．A mouse model of chemotherapy induced pain was established by intraperitoneal injection of OXA for 5 consecutive days．Intrathecal administration of lycorine was performed．Behavioral changes and expression levels of inflammatory related proteins were detected .
Results :Compared with control group，model group mice exhibited the increased number of spontaneous flinches，decreased mechanical nociceptive threshold，decreased movement distance and latency，and up-regulated expression levels of interleukin-1 β (IL-1 β) ，astrocytic marker glial fibrillary acidic protein ( GFAP) ，cyclooxygenase-2( COX-2) ，NOD-like receptor protein 3 ( NLRP3 ) ，cysteinyl aspartate and specific proteinase 1 ( Caspase- 1) ．Compared with model group，lycorine administration reduced the number of spontaneous flinches，increased mechanical nociceptive threshold ，enhanced the movement distance and latency ，bound and reduced COX-2 expression，down-regulated the expression levels of IL-1 β , GFAP ，NLRP3 and Caspase-1．
Conclusion:Lycorine reduces COX-2 expression，inhibits NLRP3 inflammasome activation，suppresses spinal inflammation，consequently alleviates pain behaviors and improved motor ability of mice.
- Full text:2024071619583608716石蒜碱降低COX-2_NL...信号缓解小鼠奥沙利铂化疗痛_过琛璐.pdf
