Overexpression of ephrinB2 in canine periodontal ligament stem cells regulates osteogenic differentiation

Yumiao Liu; Jinxin Yang; Shaoyue Zhu; Yulin Ma; Hanping Xia; Meng Zhou; Shulei Yang; Taining Huang; Kexin Ding; Zongxiang Liu

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Overexpression of ephrinB2 in canine periodontal ligament stem cells regulates osteogenic differentiation

Author: Yumiao Liu ¹ ; Jinxin Yang ² ; Shaoyue Zhu ³ ; Yulin Ma ¹ ; Hanping Xia ¹ ; Meng Zhou ² ; Shulei Yang ¹ ; Taining Huang ¹ ; Kexin Ding ² ; Zongxiang Liu ^{1
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Author Information

1. Xuzhou Medical University，Xuzhou 221004
2. Dept of Medical，The Affiliated Stomatological Hospital of Xuzhou Medical University，Xuzhou 221002
3. Dept of Science and Education， The Affiliated Stomatological Hospital of Xuzhou Medical University，Xuzhou 221002
Publication Type:Journal Article
Keywords: cPDLSCs; ephrinB2; gene transfection; osteogenic differentiation
From: Acta Universitatis Medicinalis Anhui 2023;58(3):423-428
CountryChina
Language:Chinese
Abstract: Objective:Investigate osteogenic differentiation of canine periodontal ligament stem cells ( cPDLSCs) via over-expression ephrinB2 in cPDLSCs．
Methods : cPDLSCs were isolated from the premolars and molars of Beagle．After transfected with EfnB2-GFP-Bsd and GFP-Bsd empty Vector，cPDLSCs were induced to osteogenic differentiation．Western blot was used to invest the expression of ephrinB2 protein．The effect of osteogenic differentiation of EfnB2-cPDLSCs and Vector-cPDLSCs were analyzed by RT-PCR , CCK-8，Alizarin-red S staining and ALP.
Results:There was no significant difference in cell proliferation between EfnB2-cPDLSCs and Vector-cPDLSCs．While EfnB2-cPDLSCs displayed an enhanced ALP activity and more prominent mineralized nodules compared with Vector-cPDLSCs．The odonto-/ osteogenic genes in EfnB2-cPDLSCs were also highly enhanced．
Conclusion:The results of our study indicated that ephrinB2 gene-transfected cPDLSCs showed enhanced osteogenic differentiation.
Full text:2024071619531257793过表达ephrinB2调控犬牙周膜干细胞的成骨能力_刘雨苗.pdf