The mechanism of resveratrol promoting FNDC5 degradation in skeletal muscle of male obese mice
10.19405/j.cnki.issn1000-1492.2023.03.011
- Author:
Fangmei Yu
1
;
Qiongqiong Cao
1
;
Dongmei Xu
1
;
Yueming Long
1
;
Heng Zhou
1
;
Li Gui
2
;
Yunxia Lu
1
Author Information
1. Dept of Biochemistry and Molecular Biology，School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
2. Comprehensive Laboratory， School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
resveratrol;
Sirtuin1;
Sirtuin2;
skeletal muscle;
fibronectin type Ⅲ domain-containing 5;
autophagy
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):412-417
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of resveratrol promoting fibronectin type Ⅲ domain-containing 5 (FNDC5) degradation in skeletal muscle of male obese mice．
Methods:Six-week-old male C57BL /6 mice were randomly divided into three groups : standard control diet ( SCD) ，high-fat diet ( HFD) and high-fat diet treated with resveratrol (HFD + RES) ．HFD + RES group was intervened with resveratrol via gavage ［400 mg / kg · d) ］ while fed HFD for 20 weeks．The body mass，serum TG，TC，LDL-C and HDL-C levels were detected．The pathological changes in skeletal muscle were detected by HE staining．The expression of FNDC5，SIRT1，SIRT2，LC3， p62，Beclin-1，ATG5，ATG7 was assessed by immunohistochemistry，RT-PCR and Western blot respectively.
Results:The body mass ，serum TG ，TC and LDL-C levels increased significantly ，meanwhile HDL-C levels decreased in HFD group．Lipid deposition between skeletal muscle fibers were obvious in HFD group．The immuno- histochemistry results showed that protein expression levels of SIRT1，SIRT2 and LC3 obviously decreased，while the protein levels of FNDC5 and p62 obviously increased．The expression levels of FNDC5 significantly increased， while the gene expression levels of SIRT1，SIRT2，LC3，Atg7 and Beclin-1 obviously decreased．All these responses were attenuated by treatment with RES．
Conclusion:RES has obvious effects of lipid-lowering and promoting FNDC5 degradation in skeletal muscle tissues，which may be related with SIRT1 and SIRT2-induced autophagy， thus resulting in degradation of FNDC5 .
- Full text:2024071619340861380白藜芦醇促进肥胖小鼠骨骼肌中FNDC5降解的机制_虞芳梅.pdf
