Effects of TAMs and CXCL1 on the biological behavior of hepatocellular carcinoma cell line Huh7
10.19405/j.cnki.issn1000-1492.2023.03.009
- Author:
Huiyong Zhao
1
;
Sheng Wei
1
;
Minghao Yang
1
;
Zicheng Guo
1
;
Yongfan Liu
1
;
Xiao Cui
1
Author Information
1. Dept of General Surgery，The Second Affiliated Hospital of Anhui Medical University，Hefei 230001
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
tumor-associated macrophages;
tumor microenvironment;
CXCL1;
epithelialmesenchymal transition
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):398-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects CXCL1 derived from TAMs on the progression of HCC by CXCL1 in tumor microenvironment (TME) ．
Methods :PMA induced human monocyte (THP-1) to obtain undifferentiated macrophages (M0) and then co-cultured with Huh 7 HCC cells．The biomarkers of macrophage phenotype and mRNA level of HCC were analysed by qRT-PCR. Colony formation assay，cell viability assay and transwell assay were performed to evaluate the biological alteration of HCC cells in TME．Epithelial-mesenchymal transition (EMT) molecular genetics were detected using western blot．The levels of CXCL1 in TME were measured by ELISA assay.
Results :High CXCL1 expression in HCC patients predicted poor prognosis．Abundant macrophages were found in CXCL1 high expression HCC tissues．In macrophages and HCC co-cultured model，significantly increasing CXCL1 expression was detected in both two cells and the biomarkers of M2 macrophages，CD163 and CD206 were elevated．The growth and migration of HCC cells were promoted．
Conclusion :Co-culture of macrophages with HCC cells induces macrophages pro-tumor phenotype and CXCL1 secretion which promotes the progression of HCC.
- Full text:2024071619184143662肿瘤相关巨噬细胞及其分泌的...系Huh7生物学行为的影响_赵慧咏.pdf
