Mechanism of glycyrrhetinic acid on inhibiting malignant biological behaviors of melanoma by Wnt / β - catenin pathways
10.19405/j.cnki.issn1000-1492.2023.03.008
- Author:
Yuan Li
1
;
Chong Ma
2
;
Lei Ma
1
;
Jing Wang
1
;
Chunmei Shu
1
;
Haiyou Hu
1
;
Na Li
1
Author Information
1. Dept of Dermatology and Venereology，Hernia and Abdominal Wall Surgery，Affiliated Hospital of Binzhou Medical College，Binzhou 256600
2. Dept of Colorectal Anus， Hernia and Abdominal Wall Surgery，Affiliated Hospital of Binzhou Medical College，Binzhou 256600
- Publication Type:Journal Article
- Keywords:
glycyrrhetinic acid;
melanoma;
invasion ability;
migration ability;
Wnt / β-catenin
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):392-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the possible mechanism of glycyrrhetinic acid on inhibiting malignant biological behaviors of melanoma by Wnt / β-catenin pathways．
Methods:The melanoma cells B16-F10 were selected as the research objects．The concentration gradient tests (0，1，2，4 μmol /L) were conducted by MTT．The cells given cisplatin intervention was enrolled as positive controls．The cells invasion and migration were detected by Transwell chamber assay．The expression levels of Wnt / β-catenin pathway proteins，invasion and migration related proteins (MMP-2，MMP-9) in B16-F10 cells were detected by Wester blot．The xenograft models of nude mice were constructed，and they were divided into control group (without drugs treatment) and glycyrrhetinic acid group (40 mg / kg) ．The growth of tumor tissues，and expression levels of Wnt / β-catenin pathway proteins，invasion and migration related proteins were observed.
Results :MTT results showed that glycyrrhetic acid could inhibit the proliferation of B16-F10 cells in a concentration-dependent manner．The inhibition effect of glycyrrhetic acid ( ≥2 μmol /L) was significant on the proliferation of B16-F10 cells (P ＜0. 05) ．The results of Transwell chamber assay showed that compared with control group，invasion and migration abilities of B16-F10 cells were significantly reduced after treatment with glycyrrhetinic acid (2，4 μmol /L) (P ＜0. 05) ．Wester blot results showed that compared with those without glycyrrhetinic acid treatment，expression levels of MMP-9 ，MMP-2，Wnt1 and β-catenin protein in B16- F10 cells significantly decreased after treatment with glycyrrhetinic acid (2，4 μmol /L) (P＜0. 05) ．The results of tumor-bearing assay showed that compared with control group ，weight and volume of tumors significantly decreased in glycyrrhetinic acid group，and expression levels of Wnt1 ，β-catenin，MMP-9 and MMP-2 proteins also significantly decreased (P＜0. 05) ．
Conclusion :Glycyrrhetinic acid can significantly inhibit the malignant biological behaviors of melanoma in vitro and vivo．And its mechanism may be related to inhibiting the activation of Wnt / β-catenin signaling pathways.
- Full text:2024071619120416617甘草次酸通过Wnt_β-c...瘤恶性生物学行为的机制研究_李媛.pdf
