Inhibition of ADAMTS by iPSC⁃MSCs in vitro protects cartilage matrix in patients with osteoarthritis
10.19405/j.cnki.issn1000-1492.2023.09.014
- Author:
Feng Zhang
1
;
Gang Cheng
1
;
Yujiao Wu
1
;
Zhuping Chu
1
;
Xulei Wang
1
;
Huimin Wang
1
;
Kang Wang
1
;
Wei Wei
1
;
Shangxue Yan
1
Author Information
1. Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory ofAnti⁃Inflammatory and Immune Medicine , Ministry of Education , Anhui Collaborative Innovation Centre for Anti⁃inflammatory and Immune Drugs , Hefei 230032
- Publication Type:Journal Article
- Keywords:
osteoarthritis;
iPSC derived MSCs;
articular cartilage;
a disintegrin and metalloproteinase with throm⁃ bospondin motifs
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1522-1528
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the protective effect and mechanism of iPSC⁃MSCs on cartilage matrix in knee oste⁃
oarthritis (KOA) patients in vitro.
Methods :Cartilage tissues removed from KOA patients with joint replacement surgery were collected and subjected to tissue and cellular experiments , respectively. Cartilage tissue was cut into small pieces and randomly divided into a control group , an IL⁃1β ( 10 ng/ml) induction group , and iPSC⁃MSCs 96 h and then co⁃cultured with different amounts of iPSC⁃MSCs ( 1 × 104 , 1 × 105 , 1 × 106 ) cells for 72 h. For in tissues , the pathological changes of isolated cartilage tissues were examined by HE staining. The levels of ADAMTS⁃4 , ADAMTS⁃5 , and type II collagen expression were analyzed by immunohistochemistry , while the levels of MMP13 , IL⁃6 , and IL⁃10 in culture supernatants were detected by ELISA kits. The 2 to 5 generations of chondrocytes , which were extracted from cartilage tissue of KOA patients , were stimulated with IL⁃1β ( 10 ng/ml) for 48 h and then co⁃cultured with different concentrations of iPSC⁃MSCs ( 1 × 104 , 1 × 105 , 1 × 106 ) cells for 72 h. Immunofluorescence and Western blot detected the expression of RUNX2 , ADAMTS⁃4 , and ADAMTS⁃5 in chondrocytes.
Results :Comparison with the control group , in the IL⁃1β⁃induced group , the levels of RUNX2 , ADAMTS⁃4 , and ADAMTS⁃5 increased , the level of type II collagen decreased , the levels of MMP⁃13 and IL⁃6 in the culture supernatant increased ( P < 0. 05) , and the level of IL⁃10 decreased ( P < 0. 05) ; Compared with the IL⁃1β⁃induced decreased the expression of RUNX2 , ADAMTS⁃4 , and ADAMTS⁃5 , promoted type II collagen expression and elevated IL⁃10 levels.
Conclusion :iPSC⁃MSCs inhibited ADAMTS⁃4 and ADAMTS⁃5 expression in vitro , reduced cartilage extracellular matrix degradation , and played a role in articular cartilage protection.
- Full text:2024071619055748832iPSC-MSCs体外抑制...S保护骨关节炎患者软骨基质_张峰.pdf
