LOXL1 dynamic expression of the liver in mice infected with Schistosomajaponicum
10.19405/j.cnki.issn1000-1492.2023.03.007
- Author:
Bingxin Dai
1
;
Li Gong
1
;
Xuhan Yang
1
;
Jijia Shen
1
;
Miao Liu
1
Author Information
1. Dept of Microbiology and Parasitology，Anhui Provincial Laboratory of Pathogen Biology and Anhui Key Laboratory of Zoonoses，School of Basic Medicine，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
Schistosomajaponicum;
LOXL1;
liver fibrosis;
collagen crosslinking
- From:
Acta Universitatis Medicinalis Anhui
2023;58(3):386-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To observe the dynamic expression of recombinant lysyl oxidase like protein 1 ( LOXL1) in the lysine oxidase family in the liver of C57BL/6 mice infected with Schistosomajaponicum and explore its role in hepatic fibrosis．
Methods:Mice were infected subcutaneously with cercariae of S．japonicum，and sacrificed with euthanasia in 6，9 and 12 weeks after infection．The sera and liver tissues were collected．The levels of liver fibrosis in mice was dynamically evaluated by HE and Sirius red staining，and the serum transaminases were detected．The dynamic expression levels of collagen type Ⅰ ( Col1) ，LOXL1 and α-smooth muscle actin( α-SMA) in liver tissues were determined respectively by Western blot and qPCR. Finally，the dynamic levels of soluble and insoluble collagens were detected.
Results:The result of HE and Sirius red staining showed that hepatic fibrosis levels increased at 6 weeks，peaked at 9 week，and decreased at 12 week in response to S．japonicum infection．Western blot and q-PCR showed that the expression levels of LOXL1，Col1 α1，Col3 α1 and α-SMA was significantly up regulated and reached maximum at the 9th week in response to S．japonicum infection．Soluble collagen protein levels reached maximum at the 9th week．and decreased at 12 week，however insoluble collagen protein levels continued to increase．
Conclusion:There may be a correlation between LOXL1 and fiber cross-linking in the process of hepatic fibrosis in S．japonicum，and it plays a role in promoting hepatic fibrosis.
- Full text:2024071619043845580赖氨酰氧化酶样蛋白1在日本...虫感染小鼠肝脏中的动态表达_戴冰心.pdf
