Lin28 overexpression promotes proliferation and inhibits osteogenic differentiation of human dental pulp stem cells in part through the mTOR signaling pathway
10.19405/j.cnki.issn1000-1492.2023.09.012
- Author:
Yuxi Zou
1
;
Jing Sun
2
;
Yu Sun
1
;
Xi Suo
1
;
Wenkai Zhou
1
;
Jianguang Yang
1
,
3
;
Yan Liu
1
,
3
Author Information
1. Xuzhou Medical University, Xuzhou 221004
2. School and Hospital of Stomatology, Cheeloo College ofMedicine ,Shandong University, Jinan 250014
3. Afiliated Stomatological Hospital of Xuzhou Medical University,Xuzhou 221002
- Publication Type:Journal Article
- Keywords:
Lin28;
mTOR;
human dental pulp stem cells;
proliferation;
osteogenic differentiation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1507-1513
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of Lin28 overexpression on the proliferation and osteogenic differentiation of human dental pulp stem cells (hDPSCs) through mTOR signaling pathway.
Methods :After transfecting lentiviral vectors of Lin28 gene in hDPSCs , the relative expression of Lin28 was detected by Real⁃time PCR. CCK⁃8 assay was applied to detect the effect on cell proliferation. qRT⁃PCR was used to research the expression levels of alkaline phosphatase (ALP) , osteopontin (OPN) and osteocalcin (OCN) . Western blot assay was processed to investigate the effects on the relative expression levels of ALP and OPN proteins. Alizarin red staining was utilized to detect the mineralized nodules.
Results :Compared with the control group , the cell proliferation of transfection group was promoted (P < 0. 05) ; The mRNA and protein expression levels of ALP , OPN and OCN in transfection group were significantly lower than those in control group (P < 0. 05) , the expression level of ALP apparently decreased after the addition of mTOR inhibitor rapamycin (P < 0. 05) ; Alizarin red staining showed that the size and number of mineralized nodules formed in transfection group were markedly declined compared with empty carrier group (P < 0. 05) .
Conclusion :Overexpression of Lin28 can inhibit the osteogenic differentiation of hDP⁃
SCs through suppress mTOR signaling pathway.
- Full text:2024071618552226254Lin28过表达部分通过m...干细胞的增殖并抑制成骨分化_邹雨希.pdf
