A comparative study of the effects of psoriasis serum and M5 factors on keratinocyte inflammation and proliferation
10.19405/j.cnki.issn1000-1492.2023.09.009
- Author:
Zehong Wang
1
;
Junqin Li
2
;
Peng Zou
1
;
Xinhua Li
2
Author Information
1. Dept of Microbiology and Immunology , School of Basic Medical Sciences , Shanxi Medical University , Jinzhong 030619
2. Dept of Dermatology , Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis , Taiyuan Central Hospital of Shanxi Medical University , Taiyuan 030009
- Publication Type:Journal Article
- Keywords:
psoriasis;
keratinocytes;
inflammation;
proliferation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1488-1492
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the differences in the effects of psoriatic serum and a mixture of five proinflam⁃ matory cytokines on keratinocyte inflammation and proliferation .
Methods :he human immortalized keratinocyte cell line (HaCaT cells) was used to cultivate with serum of healthy human serum , psoriasis patients and M5 factors , and the cell proliferation was monitored by CCK⁃8 . RT⁃qPCR was used to detect the mRNA expression levels of inflammatory factors interleukin 1β/8/21 /23 (IL⁃1β/8/21 /23) , proliferation markers keratin 6/16 (K6/K16) and nucleus related antigen 67 (Ki67) in HaCaT cells in each group .
Results : Healthy human serum , psoriasis serum and M5 factors could effectively promote the growth of HaCaT cells , and psoriasis serum and M5 factors could promote cell growth more than healthy human serum . Similar to M5 factors , serum from patients with psoriasis significantly promoted the mRNA levels of inflammatory factors IL⁃1β , IL⁃8 , IL⁃23 and proliferation markers K6 and Ki67 in cells . Different from M5 factors , the serum of psoriasis patients had enhanced the mRNA level of inflammatory factor IL⁃21 , but weakened the promotion of proliferation marker K16 .
Conclusion :The serum micro environment of patients with psoriasis can promote the proliferation and inflammatory response of keratinocytes higher than that of healthy people , and is similar to M5 factors , which can be used to build a keratinocyte model of psoriasis .
- Full text:2024071618132790686银屑病血清与M5因子对角质...胞炎症和增殖影响的比较研究_王泽洪.pdf
