Effects of plasma exosome⁃derived miR⁃29b⁃3p on myocardial cell inj ury in hypoxia/reoxygenation after sevoflurane postconditioning via targeting IGF1
10.19405/j.cnki.issn1000-1492.2023.09.004
- Author:
Fangfang Deng
1
;
Jiyong Li
1
;
Li Zhang
1
;
Gaorui Zou
1
;
Zhijun Chen
1
;
Wei Le
2
Author Information
1. Dept of Anesthesia, Wuhan No. 1 Hospital , Wuhan 430022
2. Dept ofAcupuncture and Moxibustion , Wuhan No. 1 Hospital , Wuhan 430022
- Publication Type:Journal Article
- Keywords:
plasma exosomes;
miR⁃29b⁃3p;
IGF1;
sevoflurane;
cardiomyocyte injury
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1450-1457
- CountryChina
- Language:Chinese
-
Abstract:
Objective :The purpose of this study was to investigate the effects of plasma exosome⁃derived miR⁃29b⁃3p in myocardial ischemia⁃reperfusion injury ( MIRI) rats on hypoxia/reoxygenation ( H/R) cardiomyocyte after
sevoflurane (SEV) postconditioning through targeting IGF1 .
Methods : The GEO database was used to screen differentially expressed miRNAs in MIRI , and cardiomyocytes were treated with H/R to construct a MIRI cell model. The expression of miR⁃29b⁃3p and IGF1 in the MIRI cell model post⁃treated with SEV was intervened , and then the survival rate of cardiomyocytes was detected by MTT , apoptosis was detected by flow cytometry , and inflammatory factors (IL⁃1β and TNF⁃α ) in cardiomyocytes in each group were detected by ELISA.
Results :Compared with Normal group , the expression of miR⁃29b⁃3p in plasma exosomes of MIRI rats was enhanced (P < 0. 05) , and the target⁃binding relationship between miR⁃29b⁃3p and IGF1 was confirmed ( P < 0. 05) . After SEV post⁃treatment , the expression of miR⁃29b⁃3p in H/R⁃stimulated cardiomyocytes decreased , while the expression of IGF1 increased (both P < 0. 05) . Overexpression of miR⁃29b⁃3p in plasma exosomes could significantly inhibit the survival rate of H/R cells after SEV treatment , aggravate apoptosis and inflammatory response , while knockdown of miR⁃29b⁃3p showed a opposite effects (all P < 0. 05) . The rescue experimental data showed that overexpression of IGF1 could partially reverse the effects of overexpression of miR⁃29b⁃3p on H/R cell injury after SEV treatment ( all P <0. 05) .
Conclusion :Plasma exosome⁃derived miR⁃29b⁃3p promotes H/R cardiomyocyte injury after SEV treatment by targeting IGF1 .
- Full text:2024071617473966735血浆外泌体源性miR-29...氧_复氧心肌细胞损伤的作用_邓方方.pdf
