AGO and RDRP genes are involved in the stress response of Aspergillus flavus

Xiang Liu; Bi Yang; Xun Tian; Jianhong Zhou; Yonghui Liao; Lingling Liu; Wenfeng Yu; Xiaolan Qi; Yinhui Jiang

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AGO and RDRP genes are involved in the stress response of Aspergillus flavus

Author: Xiang Liu ^{1
,

2
,

3} ; Bi Yang ^{1
,

2
,

3} ; Xun Tian ^{1
,

2
,

3} ; Jianhong Zhou ^{1
,

2
,

3} ; Yonghui Liao ^{1
,

2
,

3} ; Lingling Liu ^{1
,

2
,

3} ; Wenfeng Yu ^{1
,

2
,

3} ; Xiaolan Qi ^{1
,

2
,

3} ; Yinhui Jiang ^{1
,

2
,

3}
Author Information

1. Key Laboratory of Medical Molecular Biology of Guizhou Province , Guizhou Medical University , Guizhou 550004
2. Guiyang &
3. Key Laboratory of Endemic and Ethnic Diseases , Ministry of Education , Guizhou 550004
Publication Type:Journal Article
Keywords: Aspergillus flavus; RNAi; stress response
From: Acta Universitatis Medicinalis Anhui 2023;58(9):1442-1449
CountryChina
Language:Chinese
Abstract: Objective : To explore the role of Argonaute ( Ago) gene and RNA⁃Dependent RNA Polymerase (RDRP) gene of Aspergillus flavus in the growth and development about the RNAi mechanism .
Methods : A. flavus Ago1 , Ago2 , RDRP1 , RDRP3 gene mutant strains were constructed by homologous recombination . The growth and development of the mutant strains were observed on potato dextrose agar(PDA) + uracil uridine (UU) medium inoculated with 3 μl 106 CFU/mL spores . 200 , 400 μg cell wall pressure agent conidored ( CR) , 0. 8 mol/L , 1 . 6 mol/L osmotic pressure agent NaCl , 2 mmol/L , 4 mmol/L oxidative pressure agent hydrogen peroxide (H2 O2 ) and 0. 01% , 0. 02% genomic damage agent methyl mesylate (MMS) were added to the Yeast extract Glucose Minimum (YGM) + UU medium to analyze the stress response of the mutant strains .
Results :A. flavus mutant strains about ΔAgo1 , ΔAgo2 , ΔRDRP1 , ΔRDRP3 were successfully constructed and its growth and development were normal . The ΔAgo1 and ΔAgo2 strains reduced the stress effects on cell wall and osmotic pressure compared to the control . Ago1 gene deletion reduced the effect of H2 O2 , and conversely RDRP3 gene deletion increased the inhibition of H2 O2 . The Ago2 and RDRP1 strains reduced the effect on genetic damage agent . In addition , ΔRDRP1 increased the effect of osmotic stress .
Conclusion : The Ago1 , Ago2 , RDRP1 and RDRP3 genes of A. flavus are not in⁃ volved in the regulation of growth rate and asexual reproduction and can participate in the regulating of the host stress response to the environment .
Full text:202407161742030684AGO和RDRP基因参与黄曲霉压力应激反应_刘翔.pdf