Population genetics analysis of Oncomelania hupensis in Jiaxing， Zhejiang Province， 2022
10.19428/j.cnki.sjpm.2024.23536
- VernacularTitle:2022年浙江省嘉兴市湖北钉螺的群体遗传学分析
- Author:
Weiling GU
1
;
Hanqi PENG
2
;
Hanxiang ZHANG
2
;
Zelin XIANG
1
;
Zhongwen CHEN
1
;
Xiaofei FU
1
;
Yunpeng QI
1
;
Liang XIE
1
;
Jie HU
1
;
Dabing LYU
2
Author Information
1. Department of Gastroenterology, The First Hospital of Jiaxing City, Jiaxing, Zhejiang 314050, China
2. Department of Epidemiology and Statistics, School of Public Health, Soochow University, Suzhou, Jiangsu 215127, China
- Publication Type:Journal Article
- Keywords:
Oncomelania hupensis;
microsatellite marker;
population genetics;
demographic history;
Jiaxing City
- From:
Shanghai Journal of Preventive Medicine
2024;36(6):559-562
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo genotype Oncomelania hupensis， based on microsatellites， in different snail-bearing environments in Jiaxing City， Zhejiang Province， for population genetics analysis in order to explore the reasons and influencing factors for the existence or proliferation of snails and to provide scientific basis for effective monitoring and control of snails. MethodsA total of 90 snail samples from three populations were collected in Yaobang Village （YB） and Sanxing Village （SX） in Pinghu City， and Yunhe Farm （YH） in Xiuzhou District， all were selected for snail checking in key snail habitats of Jiaxing City in 2022. DNA of the snails was genotyped and analyzed for population genetics using nine microsatellite loci. ResultsA total of 84 alleles were observed， and the mean number of alleles （Na） was 7.889， 5.667， and 3.778 for YB， SX， and YH respectively； the number of effective alleles （NeA） was 4.807， 3.329， and 2.294， respectively； and the coefficients of inbreeding （FIS） were 0.400， 0.377， and 0.493， respectively. Under the Infinite Allele Model （IAM）， the SX and YH might have a recent bottleneck. The NEstimator and LDNe software calculated effective population sizes （Ne） were above 31.9. AMOVA analysis showed that the variation of snails in the three populations mainly existed among individuals， accounting for 41.4% of the total variation. The value of the index of genetic differentiation between populations （FST） was 0.286， indicating a high degree of genetic differentiation. The results of the principal component analysis and phylogenetic tree were consistent， and the three populations were divided into two lineages， YB and SX were one lineage， and YH belonged to another independent lineage. Population history and dynamics analysis showed that the gene flow of the three populations was insufficient， population divergence history indicated that YH might have diverged from SX first， and YB was produced by the contact fusion of SX and YH. ConclusionThe genetic diversity of snail populations in Jiaxing City is generally low， and the snail populations are unstable， with a great degree of genetic differentiation and insufficient gene flow among populations. This study can provide a basis for evaluating the effectiveness of the control of the snail as well as monitoring the trend of the spread of the snail.
