The role of cAMP /Epac / Rap1 signaling pathway regulate the secretion of IL-1 β, TNF-α, BDNF from NG2 cell and the effect of Jujuboside A
10.19405/j.cnki.issn1000-1492.2023.02.016
- Author:
Tingting Yang
1
;
Hui Wang
1
;
Peng Shi
1
;
Liu Teng
1
;
Yue Li
1
;
Min Du
1
;
Xiaohua Tu
1
;
Guangyong Yang
1
;
Ying Deng
1
Author Information
1. School of Basic Medicine，Guizhou University of Traditional Chinese Medicine，Guiyang 550025
- Publication Type:Journal Article
- Keywords:
NG2 cell;
Jujuboside A;
cAMP;
Epac;
Rap1
- From:
Acta Universitatis Medicinalis Anhui
2023;58(2):265-270
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether the cyclic adenosine monophosphate (cAMP) / exchange proteins directly activated by cAMP (Epac) / ras-related protein 1 ( Rap1 ) signalling pathway is involved in the intervening mechanisms of interleukin-1 β (IL-1 β) ，tumor necrosis factor-α (TNF-α) ，brain-derived neurotrophic factor (BDNF) and Jujuboside A(JuA) secretion by NG2 cells．
Methods:NG2 cells were cultured in vitro and the experiment was divided into control group ，pertussis toxin ( PTX) group ，ESI-09 group，JuA group and positive drug group．The effect of different concentrations of JuA on the survival rate of NG2 cells was detected by CCK-8 method，and the expression of IL-1 β , TNF-α , BDNF，cAMP，Epac，Rap1 mRNA and protein in each group was detected by RT-PCR and Western blot.
Results:Compared with the control group，the PTX group decreased the expression of IL-1 β and TNF-α mRNA and protein (P＜0. 01) and increased the expression of cAMP and BDNF mRNA and protein (P＜0. 01) ; the ESI-09 group increased the expression of IL-1 β and TNF-α mRNA and protein (P < 0. 05) and decreased the expression of BDNF，Epac and Rap1 mRNA and protein expression (P＜0. 01) ; the JuA group and positive drug group increased IL-1 β , TNF-α , BDNF，cAMP，Epac，Rap1 mRNA and protein expression (P＜0. 01) ．
Conclusion :The cAMP / Epac / Rap1 signaling pathway is involved in the secretion of IL-1 β , TNF- α , and BDNF by NG2 cells．JuA may act on cAMP / Epac / Rap1 signaling pathway to affect the secretion of BDNF by NG2 cells.
- Full text:2024071421542881274cAMP_Epac_Rap...F及酸枣仁皂苷A的作用研究_杨婷婷.pdf
