Establishment of the rat sepsis model by intraperitoneal injection of allogenic fecal filtrate
10.19405/j.cnki.issn1000-1492.2023.02.011
- Author:
Hui Liu
1
,
2
;
Junchao Liu
2
;
Weibao Wang
3
;
Huibo Du
4
;
Fulong Li
2
;
Chunyu Niu
5
,
6
;
Zigang Zhao
1
,
6
Author Information
1. Institute of Microcirculation / College of Basic Medicine，Hebei North University，Zhangjiakou 075000
2. Dept of Anesthesiology，The First Affiliated Hospital of Hebei North University，Zhangjiakou 075000
3. Dept of Clinical Laboratory，The Fourth Hospital of Zhangjiakou City，Zhangjiakou 075000
4. Institute of Microcirculation / College of Basic Medicine，Hebei North University，Zhangjiakou 075000
5. College of Basic Medicine，Hebei Medical University，Shijiazhuang 050017
6. Laboratory of Pathogenesis and Intervention of Acute Critical Illness of Hebei Province，Zhangjiakou 075000
- Publication Type:Journal Article
- Keywords:
sepsis;
intraperitoneal injection;
fecal filtrate;
animal model
- From:
Acta Universitatis Medicinalis Anhui
2023;58(2):236-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the method of intraperitoneal injection of allogenic fecal filtrate to establish the rat model of moderate and severe sepsis．
Methods:The preparation method of allogeneic fecal filtrate was determined．Allogeneic fecal filtrate of different concentrations (0. 5，1，2 g / kg) was injected intraperitoneally to observe the general situation，survival time and severe degree of sepsis of rats. After determining the optimal concentration，the success rate of the model，serum inflammatory factors，serum concentration of D-lactic acid ( D-LA) and serum intestinal fatty acid binding protein (I-FABP) ，lung function changes，lung，liver and kidney tissue injury were further observed.
Results:After intraperitoneal injection of allogenic fecal filtrate for 24 h，the rats of 1 g / kg group presented fever，tachypnea and hypotension，the survival rate was 83. 3% at 24 h and 16. 7% at 48 h， 2 g / kg group rats all died within 24 h，the dose of 1 g / kg was determined for subsequent experiments．Injected fecal filtrate for 24 h，the success rate of the sepsis model was 77. 8% . The levels of interleukin-6 ( IL-6) ，tumor necrosis factor-α ( TNF-α) ，D-LA and I-FABP in serum significantly increased． There were severe edema and bleeding in lung tissue，Pulmonary function appeared respiratory dysfunction，included functional residual capacity (FRC) ，quasi static compliance ( Cdyn) ，forced expiratory volume for the first 100 milliseconds(FEV100) ，peak
expiratory flow (PEF) decreased，airway resistance (RI) ，inspiratory capacity (IC) increased．Liver and kidney tissues also showed varying degrees of edema and inflammatory cell infiltration，the levels of alanine aminotransferase (ALT) ，aspartate aminotransferase (AST) ，blood urea nitrogen (BUN) and creatinine ( Cr) in serum significantly increased．
Conclusion:Intraperitoneal injection of allogenic fecal filtrate ( 1 g / kg) can produce a relative typical septic model in rats.
- Full text:2024071421040342776腹腔注射同种异体粪便滤液建立大鼠脓毒症模型_刘辉.pdf
