Dynamic pathological changes of Toxoplasma cysts in mouse brain during chronic infection
10.19405/j.cnki.issn1000-1492.2023.02.002
- Author:
Mengyun Wu
1
,
2
;
Ru Cai
3
;
Yifan Li
2
;
Xuelong Wang
2
;
Yong Yao
2
Author Information
1. Medical College of Anhui University of Science and Technology，Huainan 232001
2. Dept of Parasitology and Microbiology，School of Basic Medicine，Anhui Medical University，Hefei 230032
3. Medical College of Anhui University of Science and Technology，Huainan 232001
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
cysts of Toxoplasma gondii;
brain tissue;
pathological change;
HE dyed
- From:
Acta Universitatis Medicinalis Anhui
2023;58(2):184-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the distribution of toxoplasma cysts in the brain of infected mice and the effect of pathological changes on the behavior and neuropsychiatry of the mice during chronic infection with Toxoplasma gondii( T．gondii) ．
Methods :Mice were infected with Prugniaud strain of T．gondii by oral gavage．The brain tissues of infected mice were collected on the days of 10，30，40，90，120 and 160 after infection respectively，and the hippocampal hypothalamus，prefrontal lobe ，striatum and cerebellum regions were separated．The number of cysts and neuropathological changes in each infected area were observed and recorded by HE staining．The number of cysts and neuropathological changes in each infected area were observed and recorded.
Results :T．gondii infected mice showed symptoms of vertical hair and arched back，which were the most significant on the 40th day，and then gradually recovered with hemiplegia and circling in circles． At each time point ，the number of toxoplasma cysts was the largest in hippocampal hypothalamus，followed by prefrontal lobe and striatum，and the least in cerebellum．The diameter of toxoplasma cysts increased with time．During chronic infection，specific pathological manifestations of toxoplasma encephalitis，such as neuronophagy，were observed in all regions of the brain tissue．The above pathological changes of toxoplasma encephalitis reached the peak on the 40th day，and gradually recovered， and increased to the stimulation peak on the 120th day，and then gradually recovered．
Conclusion: The behavioral and neuropsychiatric symptoms of T．gondii during chronic infection were correlated with the localization and distribution of toxoplasma cysts in the brain of infected mice，and showed dynamic changes.
- Full text:2024071322213578925慢性感染阶段弓形虫包囊在小鼠脑组织的动态病理变化_吴梦云.pdf
