Exploring the role and mechanism of armillariella tabescens polysaccharides interference in 5-FU-induced intestinal mucosal injury based on ARRB1
10.19405/j.cnki.issn1000-1492.2023.10.023
- Author:
Quan Zhang
1
;
Qinying Zhao
1
;
Yingquan Ye
1
;
Li Liu
1
;
Mei Zhang
1
,
2
Author Information
1. Oncology Dept of Integrated Traditional Chinese and Western Medicine，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. Graduate School of Anhui University of Traditional Chinese Medicine，Hefei 230022
- Publication Type:Journal Article
- Keywords:
ARRB1;
5-fluorouracil;
intestinal mucositis;
tight junction protein;
organoid
- From:
Acta Universitatis Medicinalis Anhui
2023;58(10):1743-1749
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect and mechanism of ARRB1 on Armillariella tabescens polysaccharides reversal of 5-fluorouracil ( 5-FU ) -induced chemotherapeutic intestinal mucosal injury．
Methods : Twelve ARRB1 knockout ( ARRB1 －/ － ) and wild-type ( WT) C57BL /6 mice were randomly divided into Control，Model and ATPS groups (200 mg / kg) ，respectively．5-FU (50 mg / kg) was injected intraperitoneally for 7 days to establish a model of chemotherapeutic intestinal mucosal injury．The histopathological damage of jejunum was evaluated by HE staining ; the activity of serum superoxide dismutase (SOD) and diamine oxidase (DAO) was measured by kits ; the expression of tight junction protein (TJ) markers ZO-1，Occludin，Claudin-1 and proliferation-associated protein Ki-67 was detected by immunohistochemistry．Crypt isolation and organoid culture were used to detect the growth status of small intestinal organoids.
Results :5-FU chemotherapy reduced body weight，aggravated histopathological damage in small intestine，decreased SOD level，TJ protein and Ki-67 protein expression，increased serum DAO level，decreased spherical structure formation rate and organoid formation rate ; compared with the model group，after ATPS treatment，WT mice recovered body weight，decreased pathological damage，increased serum SOD level，TJ protein and Ki-67 protein expression，DAO levels decreased，and the rates of spherical structure for- mation and organoid formation were significantly higher．However，ARRB1 －/ － mice failed to reverse the effect of 5- FU after ATPS treatment．
Conclusion :ATPS reverses 5-FU-induced intestinal mucositis through the protective effects of ARRB1 on intestinal barrier and organoid growth.
- Full text:2024071219181223782基于ARRB1探讨亮菌多糖...导的肠黏膜损伤的作用及机制_张泉.pdf
