Luteolin inhibits the invasion and migration of endometrial stromal cells by regulating HPGD expression
10.19405/j.cnki.issn1000-1492.2023.10.017
- Author:
Yanshe Shao
1
;
Xuemei Xu
1
;
Baoqin Yang
1
;
Huijuan Li
2
;
Xia Ji
1
Author Information
1. Dept of Obstetrics and Gynecology，Henan Province Hospital of Traditional Chinese Medicine ( The Second Affiliated Hospital of Henan University of Traditional Chinese Medicine) ，Zhengzhou 450000
2. Dept of Reproductive Medicine，The First Affiliated Hospital of Henan University of Traditional Chinese Medicine，Zhengzhou 450000
- Publication Type:Journal Article
- Keywords:
endometriosis;
luteolin;
HPGD;
invasion;
migration
- From:
Acta Universitatis Medicinalis Anhui
2023;58(10):1706-1711
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of luteolin on invasion and migration of endometriosis stromal cells and whether its mechanism is related to the regulation of 15-hydroxyprostaglandin dehydrogenase (HPGD) expres- sion．
Methods :The endometrial stromal cells HEM15A were divided into control group (cells were cultured in nor- mal medium) ，luteolin group ( cells were treated with different concentrations of luteolin) ，si-HPGD group ( cells were transfected with si-HPGD) ，si-NC group ( cells were transfected with si-NC ) ，luteolin + si-HPGD Group (cells were transfected with si-HPGD and treated with 20 μmol / L luteolin) ，Luteolin + si-NC Group ( cells were transfected with si-NC and treated with 20 μmol / L luteolin) . Real-time quantitative PCR was used to detect mRNA level of HPGD．Cell proliferation was detected by CCK-8 assay，Transwell and scratch assays were used to detect cell invasion and migration．The protein expression of proliferating cell nuclear antigen (PCNA) ，matrix metallopro- teinase (MMP-2) ，MMP-9 and HPGD were detected by Western blot，and the level of prostaglandin E2 ( PGE2) was detected by ELISA.
Results :Compared with 0 μmol / L luteolin，luteolin at 20，50 and 100 μmol / L significantly inhibited the proliferation activity of hEM15A cells，and reduced PCNA expression ( all P＜0. 05) ．Compared with the control group，20 μmol / L of luteolin significantly inhibited cell invasion and migration (P ＜0. 05) ，decreased the expressions of MMP-2 and MMP-9 (P＜0. 05) ，and up-regulated the mRNA and protein levels of HPGD (P < 0. 05) ，while inhibited cellular PGE2 level (P＜0. 05) ．Compared with the luteolin group，the luteolin + si-HPGD group increased cell invasion and migration (P ＜0. 05 ) ，increased the expressions of MMP-2 and MMP-9 (P < 0. 05) ．
Conclusion :Luteolin regulates HPGD / PGE2 signaling pathway to inhibit the invasion and migration of en- dometrial stromal cells.
- Full text:2024071218535827361木犀草素调控HPGD表达抑...子宫内膜间质细胞侵袭和迁移_邵艳社.pdf
