Construction of macrophage-specific MST1 knockout mouse model
10.19405/j.cnki.issn1000-1492.2023.10.011
- Author:
Qian Xue
1
;
Jianyang Li
2
;
Wenqian Gao
1
;
Yuxia Zhang
1
Author Information
1. Dept of Pathophysiology，School of Basic Medicine，Anhui Medical University，Hefei 230032
2. First Clinical Medical Institute，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
mammalian sterile line 20-like kinase 1;
Cre-loxP;
macrophage;
gene knockout mice;
Hippo pathway
- From:
Acta Universitatis Medicinalis Anhui
2023;58(10):1672-1677
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To establish myeloid ( including macrophage and granulocyte) specific knockout mice of mammalian sterile line 20-like kinase 1 (MST1) gene for furtherinvestigating the role and the mechanism of MST1 in macrophages in related clinical diseases．
Methods :Mst1flox/flox LysM-Cre ( referred to as Mst1ΔM/ΔM hereafter) mice were generated by crossing Mst1flox/floxwith lysozyme (Lysm-Cre) mice．The loxP site and Cre gene were amplified by PCR for genotyping．The knockdown efficiency of MST1 in macrophages was verified by quantitative PCR and immunofluorescence．The main immune cell populations in the livers were detected by flow cytometry.
Results:Mst1flox/flox LysM-Cre (Mst1ΔM/ΔM ) was the genotype of macrophage specific knockout MST1 mice．The results of qPCR and immunofluorescence showed that the knock-out efficiency of MST1 was more than 70% in bone marrow- derived macrophages and peritoneal macrophages．Flow cytometry showed that macrophage knockout of MST1 had no significant effect on the main immune cell populations in the liver of mice．
Conclusion : Macrophage-specific knockout of MST1 mouse model is successfully established，which lays a foundation for further investigation on the role and mechanism of macrophage MST1 in clinical related disease.
- Full text:2024071218135029857巨噬细胞特异性敲除MST1小鼠模型的构建_薛茜.pdf
