Effects of miR-135a-5p on the expression of insulin-like factor 3 and testosterone in flutamide-induced cryptorchidism mouse
- Author:
Yuangui Yang
1
,
2
;
Nini An
1
,
2
;
Hui Chen
1
,
2
;
Guoqing He
1
,
2
;
Xiong Zhan
1
,
2
;
Moudong Wu
1
,
2
;
Dan Wang
1
,
2
;
Wei Wang
1
,
2
;
Jinpu Peng
1
,
2
Author Information
- Publication Type:Journal Article
- Keywords: cryptorchidism; flutamide; miR-135a-5p; insulin-like factor 3; testosterone
- From: Acta Universitatis Medicinalis Anhui 2023;58(10):1662-1665
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect of miR-135a-5p on the expression levels of insulin-like factor 3 (INSL3) and testosterone in the testicular tissues of flutamide-induced cryptorchidism mouse．
Methods : A model of flutamide-induced cryptorchidism in mouse was constructed，and the experiment was divided into normal control group，flutamide group，flutamide + miR-135a-5p knockdown group and flutamide + miR-135a-5p overexpression group. RT-qPCR was used to detect the expression levels of miR-135a-5p and INSL3 mRNA．Western blot was em- ployed to assess the protein expression level of INSL3．ELISA was performed to measure the expression level of tes- tosterone.
Results :The expression levels of miR-135a-5p，INSL3 mRNA and protein and testosterone were significantly down-regulated in the testis of cryptorchid mice by flutamide (P＜0. 05) ．Knockdown of miR-135a-5p could downregulate the expression of INSL3 mRNA，INSL3 protein and testosterone (P ＜0. 01 ) ，while overexpression of miR-135a-5p had the opposite result．
Conclusion :miR-135a-5p decreased in flutamide-induced cryptorchidism mouse testicular tissues，and overexpression of miR-135a-5p could restore the expression levels of INSL3 and testosterone. - Full text:2024071218031550816miR-135a-5p对氟...素样因子3及睾酮表达的影响_杨远贵.pdf
