The effect of sinomenine on proliferation and apoptosis of multiple myeloma U266 cells through regulation of STAT3 and NF-κB signaling pathways
10.19405/j.cnki.issn1000-1492.2023.10.004
- Author:
Yingying Wang
1
;
Shiying Zhou
2
;
Jie Chen
2
;
Ying Qiao
2
;
Yi Zhao
2
;
Jiayu Wu
2
;
Yingli Wu
1
;
Wei Weng
2
Author Information
1. Dept of Pathophysiology，Shanghai Jiaotong University School of Medicine，Key Laboratory of Cell Differentiation and Apoptosis，Ministry of Education，Shanghai 200025
2. Dept of Blood Transfusion，Tongren Hospital，Shanghai Jiaotong University School of Medicine，Shanghai 200336
- Publication Type:Journal Article 期刊论文
- Keywords:
resveratrol;
multiple myeloma;
U266 cells;
STAT3 signaling pathway;
NF-κB signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2023;58(10):1634-1638
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of sinomenine on proliferation and migration of multiple myeloma (MM) cells by regulating STAT3 and NF-κB signaling pathway．
Methods : The cultured U266 cells were treated with different concentrations of sinomenine (0，0．5，1，2 mmol / L) ．The control group was added DMSO with 0．5% concentration．CCK-8 assay was used to detect the proliferation of U266 cells．Flow cytometry was used to detect the apoptosis of U266 cells．Western blot assay was used to detect the expression levels of apoptosis-related proteins， STAT3 and NF-κB signaling pathway proteins in the each group.
Results : Compared with CON group，the apopto- sis of U266 cells increased after Sinomenine treatment，the proliferation was inhibited ; B lymphoma-2 (Bcl-2) mye- loid and cell leukemia-1 (Mcl-1) expression level decreased ; activated Caspase-3 (cleaved Caspase-3) and PARP (Cleaved Caspase-3) expression levels increased ; the activity of STAT3 and NF-κB signaling pathway decreased.
Conclusion : Sinomenine can down-regulate the activity of STAT3 and NF-κB signaling pathway，promote the apop- tosis of U266 cells and inhibit the proliferation of U266 cells.
- Full text:2024071217372126329青藤碱通过调控STAT3及...266细胞增殖、凋亡的影响_王莹莹.pdf
