Hypothyroidism during pregnancy in mice induces lipid dysregulation in female offspring's early life
10.19405/j.cnki.issn1000-1492.2023.10.002
- Author:
Chengcheng Li
1
;
Yan Lin
2
;
Juan Liu
2
;
Keyang Chen
1
,
3
Author Information
1. Dept of Nutrition and Food Hygiene，School of Public Health，Anhui Medical University，Hefei 230032
2. Dept of Health Inspection and Quarantine， School of Public Health，Anhui Medical University，Hefei 230032
3. Dept of Health Inspection and Quarantine， School of Public Health，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
hypothyroidism;
pregnancy;
lipid metabolism;
female offspring
- From:
Acta Universitatis Medicinalis Anhui
2023;58(10):1622-1626,1633
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To evaluate the effect of maternal hypothyroidism on lipid metabolism in the early life of emale offspring by constructing a murine model during pregnancy．
Methods : Female mice C57BL /6J were ran- domly divided into normal control group ( NC0 ) ，hypothyroidism complicated with pregnancy group ( PH0 ) and pregnancy complicated with hypothyroidism group ( GH0) ．Firstly，PH0 group was given 0．15% propylthiouracil di- et for 28 days，while NC0 and GH0 groups were given normal diet．After the successful modeling of PH0 was con- firmed，female mice in GH0 and PH0 groups were given 0．15% propylthiouracil diet during pregnancy，and NC0 group was still given normal diet．After delivery，the levels of free triiodothyronine ( FT3) ，free thyroxine ( FT4) ， thyroid stimulating hormone (TSH) and total cholesterol (TC) ，triglyceride (TG) in each group were detected at 3 weeks of age for female offspring．The pathological changes of thyroid and liver were observed by HE staining．West- ern blot detected the levels of proteins associated with lipid metabolism.
Results :Compared with the NC0 group， the levels of serum FT3 and FT4 decreased，the levels of serum TSH，serum TC，hepatic TC and TG increased in the female offspring of the PH0 and GH0 groups，and lipid deposition was observed in their livers．The expression levels of HMGCR , FAS and CD36 related to lipid metabolism in livers increased，while the expressions of CPT1A and LDL-R were down-regulated.
Conclusion : Hypothyroidism during pregnancy may lead to the disturbance of lipid metabolism in the early life of female offspring mice.
- Full text:2024071217281786184小鼠妊娠期甲状腺功能减退诱...性子代生命早期脂质代谢紊乱_李程程.pdf
