Screening of Lactobacillus that inhibit Gardnerella vaginalis and preliminary study on its bacteriostatic mechanism
10.19405/j.cnki.issn1000-1492.2023.11.027
- Author:
Rui Zhang
1
;
Aixin Ma
1
;
Junzhu Wang
2
;
Chang Li
1
;
Haixu Yin
1
;
Huihui Bai
1
;
Zhaohui Liu
3
Author Information
1. Dept of Obstetrics and Gynecology, Peking University First Hospital ,Beijing 100034
2. School of Government Management , Big Data and Public Policy Laboratory, Peking University, Beijing 100091
3. Dept of Obstetrics and Gynecology, Beijing Obstetrics and Gynecology Hospital ,Beijing 100006
- Publication Type:Journal Article
- Keywords:
bacteriostatic function;
bacteriostatic protein;
vaginal Lactobacillus;
Gardnerella vaginalis;
bacterial vaginosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1962-1965
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To select and obtain vaginal Lactobacillus strains with inhibitory effect on Gardnerella vagi⁃
nalis , and to explore the possible mechanism of inhibition of Gardnerella vaginalis by Lactobacillus vaginalis strains , so as to provide a prerequisite for the development of dominant strains for human disease treatment.
Methods :Gardnerella vaginalis and vaginal Lactobacillus strains were isolated from vaginal secretions of patients with bacterial vaginosis (BV) and healthy women , respectively. The Lactobacillus strains with the ability to inhibit the proliferation of Gardnerella vaginalis were screened by spot on lawn. Double agar diffusion method was used to compare the inhibitory effects of Lactobacillus solutions , cell⁃free culture supernatants (CFCs) and bacteria on Gardnerella vaginalis.
Results :Sixty⁃two strains of vaginal Lactobacillus strains were isolated and purified from vaginal vaginal secretions of 10 patients with bacterial vaginosis. Among the 62 strains of vaginal Lactobacillus , 18 strains could inhibit the proliferation of Gardnerella vaginalis. The vaginal Lactobacillus solutions , the CFCs , and bacteria all had inhibitory effect on Gardnerella vaginalis. The inhibitory effects of vaginal Lactobacillus solutions were higher than those of the CFCs. After the CFCs were treated with proteinase K , the inhibitory effect of eight samples disappeared , and that of 10 samples weakened obviously.
Conclusion :In this paper, 62 vaginal Lactobacillus strains are purified and identified , of which 18 strains can inhibit the proliferation of Gardnerella vaginalis. The production of antimicrobial protein may be involved in the mechanism that vaginal Lactobacillus inhibits the proliferation of Gardnerella vaginalis.
- Full text:2024071217032561373抑制阴道加德纳菌的乳杆菌的筛选及其抑菌机制的研究_张瑞.pdf
