Study on the difference of gut microbiota and metabolites between urban and rural adolescents
10.19405/j.cnki.issn1000-1492.2023.11.025
- Author:
Xuhang Shen
1
;
Shiyu Su
2
;
Jing Guan
1
;
Jilong Shen
2
;
Xi Chen
1
Author Information
1. Dept of Gastroenterology, The First Afiliated Hospital of Anhui Medical University,Hefei 230022
2. The Key Laboratory ofMicrobiology and Parasitology Anhui , Anhui Provincial Laboratory ofPathogen Biology, Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
urban and rural population;
adolescent;
gut microbiota;
16S rDNA;
LC⁃MS;
metabonomics
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1952-1956
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the differences in gut microbiota and metabolites between urban and rural
middle school students and explore their significance in gut homeostasis , so as to establish a healthy lifestyle and diet for children.
Methods :Fecal samples were collected from middle school students in Hefei ( n = 14) and Jixi
county ( n = 18 , Southern Anhui) , aged 13. 0 - 13. 5 years. Stool samples were sequenced by 16S ribosomal DNA (LC⁃MS) , followed by bioinformatic analysis.
Results : Lachnoclostridium and Anaerostipes were dominant in the urban students that had been reported to be associated with colorectal cancer, atherosclerosis , depression and other disorders. In the village children , Ruminococcaceae UCG⁃002 , Barnesiella and Eubacterium dominated. An increased proportion of these microbes were related to metabolism of bile acids , short⁃chain fatty acids , lipid and carbohydrate decomposition , and play an important role in maintaining immune balance and physiological function. Additionally , significant differences in gut metabolites of the two groups were noted , mainly in arachidonic acid metabolism , platelet activation , serotonin metabolism , vitamin absorption , primary bile acid metabolism and other pathways.
Conclusion :Adolescent students of urban and mountainous areas differ in gut microbiota and metabo⁃
lites. Rural children have a healthy bacterial flora and metabolites in guts due to a reasonable lifestyle and diet in comparison with the city children.
- Full text:2024071216471832790城市与山村青少年肠道菌群及其代谢组学差异的研究_沈续航.pdf
