Study on the mechanism of a pyroptosis of renal podocytes in hypoxia and high glucose environment
10.19405/j.cnki.issn1000-1492.2023.11.019
- Author:
Zhimin Meng
1
;
Chuanchuan Liu
2
;
Yaya Ji
3
;
Qing Zhu
3
;
Fengjiao Yin
3
;
Ruixia Zhang
1
;
Yinggui Ba
4
Author Information
1. Dept of Endocrinology , Afiliated Hospital of Qinghai University, Xining 810001
2. Qinghai University High Altitude Medical Research Center , Xining 810001
3. Graduate School of Qinghai University, Xining 810001
4. Dept of Nephrology , Afiliated Hospital of Qinghai University , Xining 810001
- Publication Type:Journal Article
- Keywords:
hypoxia;
high glucose;
podocyte;
NLRP3;
pyroptosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1916-1920
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of hypoxia , high glucose single factor and hypoxia high glucose
compound factors on the pyroptosis of rat glomerular podocytes .
Methods :Rat glomerular podocytes were cultured in vitro and randomly divided into control group , high glucose group , hypoxia group and hypoxia high glucose group , EdU method was used to detect the cell proliferation , transmission electron microscope was used to observe the morphology and size changes of nucleus and mitochondria , and Western blot was used to detect pyroptosis related proteins nucleotide⁃binding oligomerization domain⁃like receptor protein 3(NLRP3) , Cysteinyl aspartate specific proteinase⁃1(Caspase⁃1) , gasdermin( GSDMD) and inflammatory factor pro⁃interleukin⁃1β( Pro⁃IL⁃1β) , interleukin(IL) Ⅳ1β , IL⁃18 . The effect of hypoxia and high glucose on the pyroptosis of rat glomerular podocytes was analyzed .
Results :EdU results showed that hypoxia and high glucose inhibited the proliferation ability of rat glomerlar podocytes (P < 0. 05) . The results of transmission electron microscopy suggested that hypoxia and high glucose promoted the occurrence of pyroptosis of rat glomerular podocytes . Western blot showed that hypoxia and high glucose promoted pyroptosis of rat glomerular podocytes , and increased the expression of pyroptosis related proteins NLRP3 , Caspase⁃1 and GSDMD , among which the expression of pyroptosis protein increased most significantly in hypoxia and high glucose group (P < 0. 05) . At the same time , it also increased the expression of pro⁃inflammatory factor Pro⁃IL⁃1β , IL⁃1β and IL⁃18 (P < 0. 05) .
Conclusion :hypoxia and high glucose can induce pyroptosis of rat glomerular podocytes , one of the mechanisms may be through affecting NLRP3 ⁃Caspase⁃1 ⁃GSDMD and its down⁃ stream inflammatory factors .
- Full text:2024071210532916228低氧、高糖环境对肾脏足细胞焦亡相关机制的研究_孟智敏.pdf
