Study on the protective mechanism of dapagliflozin on kidney in diabetic nephropathy rats
10.3969/j.issn.1673-9701.2024.10.015
- VernacularTitle:达格列净对糖尿病肾病大鼠肾脏保护作用机制研究
- Author:
Yuyan YE
1
,
2
;
Peng WANG
2
,
3
;
Xia FANG
1
,
2
;
Jing YANG
1
,
2
Author Information
1. Department of Nephrology, Jinhua People&rsquo
2. s Hospital, Jinhua 321000, Zhejiang, China
3. Department of Pharmacy, Jinhua People&rsquo
- Publication Type:Journal Article
- Keywords:
Dapagliflozin;
Diabetic nephropathy;
Kidney protection;
AMPK/mTOR signaling pathway
- From:
China Modern Doctor
2024;62(10):60-63,71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of dapagliflozin on kidney and the expression of AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)signaling pathway in diabetic nephropathy(DN)rats.Methods A total of 40 SPF Wistar male rats were randomly divided into normal group,model group,low-dose group and high-dose group,with 10 rats in each group.After the DN model was successfully prepared,the rats in normal group were given normal diet + normal saline by gavage,the rats in model group was given high sugar and high fat feed + normal saline by gavage,the rats in low-dose group was given high sugar and high fat feed+1mg/(kg·d)of dapagliflozin by gavage,the rats in high-dose group was given high sugar and high fat feed+10mg/(kg·d)of dapagliflozin by gavage.Rats in each group were continuously gavaged for 12 weeks.Renal function indexes,renal pathological changes,p-AMPK and p-mTOR protein expression,collagen type Ⅰ(COL Ⅰ),collagen type Ⅳ(COL Ⅳ)and fibronectin(FN)of all groups were compared.Results Blood urea nitrogen(BUN),serum creatinine(SCr),24h urinary protein quantity,p-mTOR protein expression,COL Ⅰ,COL Ⅳ and FN levels of rats in model group,low-dose group and high-dose group were significantly higher than those in normal group,and p-AMPK protein expression was significantly lower than that of normal group(P<0.05).BUN,SCr,24h urinary protein quantity,p-mTOR protein expression,COL Ⅰ,COL Ⅳ and FN levels of rats in low-dose group and high-dose group were significantly lower than those in model group,while p-AMPK protein expression was significantly higher than that in model group(P<0.05).BUN,SCr,24h urinary protein quantity,p-mTOR protein expression,COL Ⅰ,COL Ⅳ and FN levels in high-dose group were significantly lower than those in low-dose group,and p-AMPK protein expression was significantly higher than that in low-dose group(P<0.05).Conclusion Dapagliflozin has a good kidney protection effect on DN rats,and its mechanism may be related to the regulation of AMPK/mTOR signaling pathway.
