Study on apoptosis mechanism of Jurkat cells induced by CAHB in adult acute lymphoblastic leukemia
10.3969/j.issn.1673-9701.2024.10.010
- VernacularTitle:CAHB诱导成人急性淋巴细胞白血病Jurkat细胞凋亡机制的研究
- Author:
Jia ZHANG
1
;
Ye HONG
1
;
Baoguo CHEN
1
;
Wenda LUO
1
Author Information
1. Department of Haematology, Taizhou Hospital of Zhejiang Province, Linhai 317000, Zhejiang, China
- Publication Type:Journal Article
- Keywords:
Diacetyl hexamethylene diamine;
Acute lymphoblastic leukemia;
Jurkat cell;
Apoptosis
- From:
China Modern Doctor
2024;62(10):39-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of apoptosis induced by diacetyl hexamethylene diamine(CAHB)in adult acute lymphoblastic leukemia Jurkat cells,and to provide theoretical basis for the clinical application of CAHB.Methods Annexin V+/PI-cell rate of Jurkat cells after CAHB induction was analyzed by flow cytometry.The Annexin V+/PI-cell rate was observed after treatment with caspase-9 inhibitor Z-LEHD-FMK.The expressions of apoptosis-related proteins caspase-8,caspase-9 and caspase-3 were observed by Western blotting.Results After CAHB induction,Jurkat cells were reduced in size,cell membrane crinkling,chromatin thickening,nuclear pyknosis or fragmentation,etc.Typical apoptotic bodies could be seen.CAHB induced Jurkat cell apoptosis by activating caspase-9 and caspase-3 in a dose-effect and time-dependent manner.Caspase-9 inhibitors could inhibit apoptosis induction of CAHB to a certain extent.Conclusion CAHB induced Jurkat cell apoptosis was related to caspase-9 and caspase-3 activation.
