Expression and clinical significance of FAM83H-AS1 in breast cancer based on bioinformatics analysis
10.3969/j.issn.1673-9701.2024.09.001
- VernacularTitle:基于生物信息学分析FAM83H-AS1在乳腺癌中的表达及临床意义
- Author:
Na LI
1
,
2
;
Jun YUAN
1
,
2
;
Anzhi ZHANG
1
,
2
;
Chuling HU
3
,
4
;
Maoyi XU
5
Author Information
1. Department of Pathology, Jiaxing Maternity and Child Health Hospital, Affiliated Women and Children&rsquo
2. s Hospital of Jiaxing University, Jiaxing 314051, Zhejiang, China
3. Department of Pharmacy, Jiaxing Maternity and Child Health Hospital, Affiliated Women and Children&rsquo
4. s Hospital of Jiaxing University, Jiaxing 314051, Zhejiang, China
5. Department of Oncology, the First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China
- Publication Type:Journal Article
- Keywords:
FAM83H-AS1;
Breast cancer;
Immune checkpoint;
Tumor microenvironment;
Tumor mutation burden
- From:
China Modern Doctor
2024;62(9):1-6,21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression and clinical treatment and prognosis assessment significance of FAM83H-AS1 in breast cancer by bioinformatics analysis.Methods The expression of FAM83H-AS1 in various cancer types was analyzed in the Cancer Genome Atlas(TCGA)database by R language.Additionally the expression in breast cancer was examined by Gene Expression Profiling Interactive Analysis(GEPIA)online database.Survival data were downloaded from TCGA to analyze the correlation between FAM83H-AS1 expression levels and prognosis among breast cancer patients.GEPIA and Kaplan-Meier Plotter were used for dual verification.Clinical information was obtained from TCGA for further analysis on the relationship between FAM83H-AS1 and clinical characteristics.And the relationship between FAM83H-AS1 and tumor microenvironment,immunodetection point-related genes and tumor mutation burden(TMB)was analyzed using R language.Gene set enrichment analysis(GSEA)was performed.Results The expression of FAM83H-AS1 was abnormal in many cancers,particularly exhibiting a significant increase in breast cancer.High expression of FAM83H-AS1 is associated with significantly reduced overall survival in breast cancer patients.Furthermore,the expression level of FAM83H-AS1 varies among breast cancer patients with different clinical stages.FAM83H-AS1 exhibits negative correlation with stromal cell score and immune cell score in breast cancer samples,and correlated with immune detection point-related gene.TMB radar map results suggest that the expression of FAM83H-AS1 in breast cancer is positively correlated with tumor mutational burden.GSEA revealed a positive correlation between the expression of FAM83H-AS1 and the function of mismatch repair.Conclusion FAM83H-AS1 is highly expressed in breast cancer,and is related to poor prognosis.Its expression correlates with clinicopathological stage,tumor microenvironment and TMB of breast cancer patients.Furthermore,FAM83H-AS1 exhibits associations with certain immune checkpoint-related genes and mismatch repair genes.These findings provide a important theoretical basis for clinical treatment,prognosis prediction and development of gene-target drugs.
