Role of PD-L1 on promoting epithelial-mesenchymal transition by activating NF-κB in endometrial cancer
10.3969/j.issn.1673-9701.2024.08.014
- VernacularTitle:PD-L1激活NF-κB促进子宫内膜癌细胞上皮间质转化的作用研究1
- Author:
Yumiao NIU
1
,
2
;
Hao HUANG
1
,
2
;
Xiaojing CHEN
1
,
2
Author Information
1. Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women&rsquo
2. s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, Zhejiang, China
- Publication Type:Journal Article
- Keywords:
Endometrial carcinoma;
programmed death ligand-1;
Nuclear factor kappa-B;
Epithelial interstitial transformation
- From:
China Modern Doctor
2024;62(8):59-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of programmed death ligand-1(PD-L1)overexpression on epithelial mesenchymal transformation(EMT)in endometrial cancer cells and its possible mechanism.Methods Ishikawa/PD-L1,a PD-L1 stable overexpression cell line constructed in the laboratory and the control Ishikawa/EV were used.The efficiency of PD-L1 overexpression was detected by qPCR and Western blot assay.The cell migration ability and invasion activity were detected by scratch assay and Transwell assay.The EMT-related protein and the phosphorylation level of nuclear transcription factor-κB(NF-κB)were detected by Western blot.Results Compared with the control group,the migration ability and invasion activity of Ishikawa/PD-L1 were significantly enhanced,and the expression of E-cadherin was significantly down-regulated,whereas vimentin,N-cadherin and p-p65 were significantly increased.Conclusion PD-L1 can promote EMT by inducing the activation of NF-κB pathway,and thus enhance the migration and invasion ability of endometrial cancer cells.
