Study on the role of NR1H4 in chronic atrophic gastritis and drug prediction based on bioinformatics
10.3969/j.issn.1673-9701.2024.04.002
- VernacularTitle:基于生物信息学探究NR1H4在慢性萎缩性胃炎及药物预测中的作用
- Author:
Xiaoting PENG
1
;
Wensu WANG
2
;
Diancheng HE
3
;
Yamei ZHAN
4
,
5
;
Shaowei YOU
3
Author Information
1. The Second Clinical Medical College, Guizhou University of Traditional Chinese Medicine, Guiyang 550002, Guizhou, China
2. Department of Cadre Healthcare, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China
3. Department of Gastroenterology, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China
4. Department of Pharmacy, Guizhou Provincial People&rsquo
5. s Hospital, Guiyang 550002, Guizhou, China
- Publication Type:Journal Article
- Keywords:
Chronic atrophic gastritis;
Bioinformatics;
NR1H4;
Differentially expressed genes;
Immune infiltration
- From:
China Modern Doctor
2024;62(4):5-10,23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differential gene expression profile and small molecule drugs for chronic atrophic gastritis(CAG)by bioinformatics technology.Methods Two gene expression samples of CAG chips(GSE27411,GSE116312)were obtained through the Gene Expression Synthesis(GEO)database,screen the differentially expressed genes(DEGs)of CAG by R language,and CAG immune-related genes were obtained for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Protein-protein interaction(PPI)network was constructed using STRING database to screen out core genes,further study on immune invasion of core genes based on GSE27411 dataset,small molecular compounds interacting with core genes were predicted,molecular docking was carried out by MOE2022,and survival analysis was carried out by GEPIA2 website.Results A total of 517 DEGs were screened out based on GEO database.GO function enrichment analysis found that it mainly involved in granulocyte chemotaxis、leukocyte chemotaxis and neutrophil chemotaxis biological processes.KEGG pathway enrichment analysis showed that it mainly involved in cytokine-cytokine receptor interaction、nuclear factor kappa B signaling pathway、interleukin-17 signaling pathway.Six key genes of NR1H4、CCK、CCL20、CXCL1、LCN2、SAA1 were obtained by PPI network,through relevant verification,NR1H4 was regarded as the core gene.Immune cell infiltration analysis showed that central memory CD8 T cell、effector memeory CD4 T cell、gamma delta T cell、natural killer T cell、neutrophil and other immune cells may be involved in the development of CAG,and the neutrophil was positively correlated with NR1H4.It was predicted that six small molecular drugs,corilagin,stigmasterol,geniposide,tangeretin,chenodeoxycholic acid and epigallocatechin 3-gallate,have good binding force with NR1H4.Conclusion The potential mechanism of CAG is preliminarily explored in this study,the key gene of NR1H4 and neutrophil may play an important role in the"inflammatory cancer transformation"process of CAG,which can provide a certain reference for the study of the"inflammatory cancer transformation"mechanism of CAG.
