Paraquat causes Alzheimer's-like pathology in rats through Wnt/β-catenin signaling-mediated Aβ production
10.3969/j.issn.1673-9701.2023.35.016
- VernacularTitle:百草枯通过Wnt/β-catenin信号通路调控Aβ产生导致大鼠阿尔茨海默病样病变
- Author:
Tao LIU
1
;
Tao SHEN
;
Yanping LI
;
Hongwen LUO
;
Wei ZHANG
Author Information
1. 云南大学附属医院急诊医学部,云南昆明 650021
- Keywords:
Paraquat;
Alzheimer's disease;
Blood-brain barrier;
Aβ42
- From:
China Modern Doctor
2023;61(35):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective The annual incidence of Alzheimer's disease in China is increasing year by year.The herbicide paraquat widely used in early China may be the factor inducing Alzheimer's disease.This study intends to analyze the mechanism of inducing Alzheimer's disease by constructing an animal model of paraquat poisoning.Methods SD rats were fed paraquat,and the integrity of blood-brain barrier was observed by Evans blue staining.The expression level of Aβ42 in brain tissue was detected by Western-blot,and the expression levels of inflammatory cytokines interleukin-1β,tumor necrosis factor-α and inducible isoform of nitric oxide synthase were detected by ELISA.Results Paraquat could significantly increase the expression level of Aβ42 in brain tissue,destroy the integrity of blood-brain barrier,and increase the expression level of GSK-3β in blood-brain barrier.Further results showed that the use of GSK-3β inhibitors significantly alleviated paraquat induced blood-brain barrier damage and Aβ42 levels.Conclusion Paraquat can damage the integrity of the blood-brain barrier by up-regulating GSK-3β,and eventually lead to paraquat entering the brain,which increases the expression level of Aβ42.
