Expression of peripheral blood NKT and NK cells in postoperative colorectal cancer patients before and after chemotherapy
- VernacularTitle:结直肠癌术后患者化疗前后外周血NKT及NK细胞表达分析
- Author:
Zhong SHI
1
;
Xiaofu YU
;
Jing ZHU
;
Meiqin YUAN
;
Wangxia LV
;
Tingting FENG
;
Haijun ZHONG
Author Information
1. 浙江省肿瘤医院腹部肿瘤内科
- Keywords:
Colorectal cancer;
Lymphocyte subsets;
NK cells;
NKT cells
- From:
China Modern Doctor
2019;57(10):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of peripheral blood lymphocyte subsets in patients with postoperative colorectal cancer before and after chemotherapy, and to evaluate the changes of immune function status. Methods 74 patients with stage Ⅱ-Ⅲ colorectal cancer after radical resection admitted in Zhejiang Cancer Hospital from January to December 2016 were enrolled. 25 non-malignant patients in the same period in our hospital were selected as the control group. Flow cytometry was used to analyze the proportion of peripheral blood lymphocyte subsets at one day before the first, sixth and eighth cycles of adjuvant chemotherapy. Results There were no differences in CD3+ (P =0.1532), CD3+CD4+ (P=0.1107), CD3+CD8+ (P=0.2576) T cells in the peripheral blood between patients with colorectal cancer after radical operation and control group (P>0.05). While CD3+CD56+NKT cells (P=0.0210) and CD3-CD56+NK cells (P=0.0045) in the patients with colorectal cancer after radical operation were significantly higher than those of the control group (P <0.05). There was no significant change in the proportion of NK and NKT cells before 6 cycles of postoperative adjuvant chemotherapy (P>0.05). There was no significant change in the proportion of NKT cells before 8 cycles (P>0.05), and the proportion of NK cells only decreased slightly (19.10±4.63 vs 20.31±4.66, P=0.046). Conclusion The proportion of NK and NKT cells in patients with colorectal cancer after radical resection was significantly higher than that in healthy people, and the expression rate remains stable after adjuvant chemotherapy.
