Preliminary discussion on using equal dilution to solve the problem that LH750 could not classify white blood cells caused by Jaundice specimen
- VernacularTitle:初步探讨等量稀释法解决黄疸标本引起的LH750仪白细胞无法分类的问题
- Author:
Jingchao CHEN
1
;
Dongming ZHOU
;
Simin LIAN
;
Suwei ZHANG
Author Information
1. 广东省汕头市中心医院检验科
- Keywords:
LH750 blood cell analyzer;
Five classifications of white blood cells;
Comparison test;
Hyperbilirubinemia
- From:
China Modern Doctor
2018;56(13):111-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the accuracy and reliability of white blood cell classifications in normal saline diluted jaundice specimen which could not be classified by blood cell analyzer. Methods Jaundice specimens which not be classified were equally diluted with normal saline and assessed in LH750. Differential count of white blood cells after dilution was compared with the result of original specimen using XN-1000 blood cell analyzer. The white blood cell classifications of diluted jaundice specimen and the original blood sample were analyzed. Results In jaundice specimens diluted by normal saline, when WBC ＜2. 0 ×109/L, minimum relative deviation was 0％ and the maximum was 9. 03％. When WBC＞2. 0xl09/L, minimum relative deviation was 0. 09％ and the maximum was 5. 21％. All the results met the requirements. Conclusion Equal dilution of normal saline could preliminarily solve the problem that white blood cells could not be classified in jaundice specimen.
