Effects of Supplementation with a Selective COX-2 Inhibitor and Vitamin C on Glomerular TGF-beta, COX-2, and Antioxidant Activity in Rats with Passive Heymann Nephritis.
- Author:
Yeon Soon JUNG
1
;
Hark RIM
;
Won MOON
;
Jongwon CHOI
;
Mee Young SOL
Author Information
1. Department of Internal Medicine, Kosin University College of Medicine, Gospel Hospital, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Membranous nephropathy;
Antioxidants;
Transforming growth factor-beta;
Cyclooxygenase 2
- MeSH:
Animals;
Antioxidants;
Ascorbic Acid;
Catalase;
Complement System Proteins;
Cyclooxygenase 2;
Cyclooxygenase 2 Inhibitors;
Epithelial Cells;
Glomerulonephritis, Membranous;
Polyethylene Glycols;
Proteinuria;
Pyrazoles;
Rats;
Reactive Oxygen Species;
Sulfonamides;
Superoxide Dismutase;
Transforming Growth Factor beta;
Vitamins;
Xanthine Dehydrogenase;
Xanthine Oxidase
- From:Korean Journal of Nephrology
2009;28(5):397-409
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: In the passive Heymann nephritis (PHN) rat model of membranous nephropathy, complement induces glomerular epithelial cell injury and proteinuria, which is partially mediated by reactive oxygen species (ROS), TGF-beta, and COX-2. In the current study, we determined the effect of a selective COX-2 inhibitor (celecoxib) and vitamin C on the enzyme system associated with ROS, TGF-beta, and COX-2 in PHN. METHODS: Four groups of rats with PHN were dosed with polyethylene glycol vehicle (P; n=4), celecoxib (COXi; n=8), vitamin C (VC; n=8), or celecoxib and vitamin C (COXi+VC; n=8) from days 7-21. Each group was then divided into 2 subgroups reflecting the day of the experiment (day-14 and -21 subgroups). RESULTS: The urine protein was significantly reduced in the VC and COXi+VC groups (subgroup day- 14) compared to the P group (p<0.05). The glomerular TGF-beta expression was reduced in the COXi+ VC group (subgroup day-21) compared to the P group (p<0.05). Glomerular COX-2 expression was increased in the COXi, VC, and COXi+VC groups compared to the P group (p<0.05). The COXi, VC, and COXi+VC groups (subgroup day-21) had decreased activity of lipid peroxide and xanthine oxidase and increased activity of xanthine dehydrogenase, superoxide dismutase, GSH-Px, and catalase. This antioxidant activity was highest in the COXi+VC group (p<0.05). CONCLUSION: Selective COX-2 inhibitors possess antioxidant effects. The combination of a COX-2 inhibitor and vitamin C was more effective than COX-2 inhibitor or vitamin C alone in increasing antioxidant activity and decreasing TGF-beta.
