Improvement of animal models of neonatal necrotizing enterocolitis
- VernacularTitle:新生鼠坏死性小肠结肠炎动物模型建立方法改进
- Author:
Bin LIU
1
;
Jingjiang YAN
;
Xiaoxia LI
Author Information
1. 山西医科大学儿科医学系
- Keywords:
Neonatal necrotizing enterocolitis;
Injury of intestine;
Animal model;
New method
- From:
China Modern Doctor
2015;53(35):22-25,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a standard and simple method of the neonatal necrotizing enterocolitis(NEC) animal model by the environment simulation equipment. Methods Seventy-five newborn mice at age of 24 hours were randomLy divided into 5 groups. Group A1 group, A2 group and A3 group were given artificial feeding, discontinuities cold/hypoxia exposure and LPS gavage. Group B was only given artificial feeding. Group C was a control group. Mice were sacrificed after being fed for 84 hours. Intestinal histopathology was scored. Results There was statistically significant between group A1, group B and group C (P<0.05). In addition, all the repeated tests were obtained a same result. Conclusion The NEC animal model established by environmental simulation experiment was coincidence of the character of patho-physiology for NEC and improved the poor repeatability disadvantage, which made the NEC model device more normal-izes. Thus, the model was very suitable to research NEC.
