The application of real-time fluorescent RT-PCR in testing norovirus in aquatic products
- VernacularTitle:应用实时荧光RT-PCR法检测水产品中诺如病毒
- Author:
Yanjin JI
1
;
Wenwen SUN
;
Zhe ZHU
;
Songtao YANG
Author Information
1. 辽宁省抚顺市疾病预防控制中心
- Keywords:
Novovirus;
Aquatic products;
Enrichment;
Fluorescent quantitative RT-PCR
- From:
China Modern Doctor
2015;(19):130-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a testing method for novovirus in aquatic product samples. Methods Optimized glycine buffer was used to dispose the homogenate of aquatic products, the concentration of concentrated virus in PEG precipitation was tested, virus RNA was extracted, and the method of fluorescent quantitative RT-PCR was applied to test novovirus. Results 72 samples were tested by an optimized method, and positive PCR products were validated by nucleotide sequencing method. The positive PCR products were validated by the nucleotide sequencing method as novovirus. Conclusion The research method can be applied to test novovirus in aquatic products. Few aquatic products in the seafood market in Fushun City are contaminated by novovirus.
