Effect of different culture systems on the zona-flee embryos of mouse
- VernacularTitle:不同体外培养方法对去透明带小鼠胚胎发育的影响
- Author:
Yaguang WANG
1
;
Yuan GAO
;
Shujian QIN
Author Information
1. 辽宁医学院人体解剖与组织胚胎学教研室
- Keywords:
Embryo culture techniques;
Embryonic development;
mWOW method
- From:
China Modern Doctor
2015;(16):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different culture methods in vitro mouse zona-free embryonic devel-opment. Methods In various stages of embryonic development rate,blastocyst rate and cell numbers of blastocysts de-veloped were measured as mWOW system (the modified “well of well” system), micro-drops single + group, micro-drops single and micro-drops group culture in vitro. The effects of different methods of were compared in vitro embryo development zona-free. Results Embryonic development rate,blastocyst rate and cell numbers of blastocysts developed were significantly higher in mWOW system culture than micro-drops single + group, micro-drops single and micro-drops group culture. Conclusion Compared with micro-drops single+group,micro-drops single and micro-drops group culture,mWOW system method is more suitable for zona-free cultured in vitro mouse embryos.
